| 光谱学与光谱分析 |
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| Study on the Reaction Mechanism of Chloroacetanilide Herbicides with Urease Using Fluorescence Spectrum and High-Performance Liquid Chromatography |
| LIU Hui-jun1，ZHAN Xiu-ming1，LI Ke-bin2，LIU Wei-ping1* |
1. Institute of Environmental Science, Zhejiang University, Hangzhou 310027, China
2. Department of Chemistry, Northwest University, Xi′an 710069, China |
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Abstract The relationship between excess thermodynamic function and binding to urease of four chloroacetanilide herbicides was studied using high-performance liquid chromatography and fluorescence spectrum. The linear relationship between the composition of mobile phase of RP-HPLC and the capacity factor of chloroacetanilide herbicides has been obtained. The excess thermodynamic enthalpy (Δ*) of acetochlor, pretilachlor, butachlor and metolachlor was determined, and the binding constant K and the number of binding sites with urease were calculated. The relationship between excess thermodynamic function and the binding constant K was suggested.
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Received: 2003-09-16
Accepted: 2003-12-28
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Corresponding Authors:
LIU Wei-ping
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| Cite this article: |
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LIU Hui-jun,ZHAN Xiu-ming,LI Ke-bin, et al. Study on the Reaction Mechanism of Chloroacetanilide Herbicides with Urease Using Fluorescence Spectrum and High-Performance Liquid Chromatography [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2005, 25(03): 463-466.
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| URL: |
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https://www.gpxygpfx.com/EN/Y2005/V25/I03/463 |
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