| 光谱学与光谱分析 |
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| X-Ray Microanalysis of the Activity of Immobilized Urease on Chitosan Membrane |
| MA Xiao-li, YAO Zi-hua* |
| College of Chemistry and Environmental Science, Hebei University, Hebei Analytical Science and Technology Key Lab, Baoding 071002, China |
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Abstract The localization of the activity of immobilized urease on chitosan membrane was studied by X-ray microanalysis. BaCl2 and urea were selected as the capture and substrate respectively. The substrate was hydrolyzed by immobilized urease to produce NH3 and CO2 in Tris-HCl buffer(pH 7.0), and the latter was captured by BaCl2 to form precipitate. The precipite was deposited on the active site of immobilized urease. It is shown that the method is practicable and reliable. The optimum condition for the localization of activity of immobilized urease was studied.
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Received: 2004-04-06
Accepted: 2004-08-06
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Corresponding Authors:
YAO Zi-hua
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| Cite this article: |
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MA Xiao-li,YAO Zi-hua. X-Ray Microanalysis of the Activity of Immobilized Urease on Chitosan Membrane [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2005, 25(03): 456-459.
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| URL: |
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https://www.gpxygpfx.com/EN/Y2005/V25/I03/456 |
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