| 光谱学与光谱分析 |
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| Effect of Pb2+ on Spectral Characteristics of DNA from Fish Intestines |
| HONG Fa-shui, CAI Shu-ming, LIU Chao, ZHENG Lei, Lü Shi-peng |
| College of Life Sciences, Suzhou University, Suzhou 215006, China |
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Abstract The interaction of Pb2+ and DNA of fish intestines was investigated using absorption spectrum, fluorescence emission spectrum, and electrophoresis. Ultraviolet absorption spectra indicated that with the addition of Pb2+, the DNA generated obvious hypochromic effect, and the absorption peak at 207 nm of DNA was blue-shifted. The fluorescence emission spectra showed that with the addition of Pb2+, the emission peak of DNA at about 419 nm did not have obvious changes, but the intensity of fluorescence reduced gradually, showing that Pb2+ has quenching effect on the DNA endogenous fluorescence. Pb2+ has 0.8 binding sites to DNA, and the fluorescence quenching of DNA caused by Pb2+ belongs to static quenching. The binding constants of binding sites are 6.08×104 L·mol-1 and 2.82×104 L·mol-1 from the Lineweaver-Burk plot. The experiments of ultraviolet and fluorescence spectra showed that exogenous Pb2+ could cause some changes to the conformation of DNA, but analysis of electrophoresis proved that exogenous Pb2+ at different concentration did not result in DNA breakage.
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Received: 2003-08-12
Accepted: 2004-02-12
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Corresponding Authors:
HONG Fa-shui
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| Cite this article: |
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HONG Fa-shui,CAI Shu-ming,LIU Chao, et al. Effect of Pb2+ on Spectral Characteristics of DNA from Fish Intestines [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2005, 25(03): 424-427.
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| URL: |
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https://www.gpxygpfx.com/EN/Y2005/V25/I03/424 |
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