| 光谱学与光谱分析 |
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| Nonradioactive Iodine-Labeled Antibodies-Inductively Coupled Plasma Mass Spectrometry for Immunoassay |
| LI Jing-xi1, WANG Xiao-ru1, 2*, ZHUANG Zhi-xia2, CUI Wei-gang1 |
1. Marine Ecology Research Center, First Institute of Oceanography of State Oceanic Administration，Qingdao 266061，China
2. Department of Chemistry, the Key Laboratory of Analytical Science of Ministry of Education, Xiamen University, Xiamen 361005, China |
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Abstract In the present study, the system of nonradioactive iodine-labeled-antibodies linking inductively coupled plasma mass spectrometry for immunoassay was reported. The goat-anti-Escherichia coli and goat anti rabbit were considered as simulant antigen and antibody respectively in order to establish a new method of immunoassay by inductively coupled plasma mass spectrometry which has the advantage of high sensitivity, low detection limit and preferable linearity range. During the experiment, the N-bromosuccinimide, a mild oxidant, was used to oxidize the non-radioactive iodine (127I) that labeled the protein. The method of nonradioactive iodine labeled protein was established and the best labeling condition was explored. The compound of I was purified by Sephadex G50 column chromatography, then the stability and activity were examined. The results showed that the labeling program was simple, reaction time was within two minutes, the labeling yield achieved 63.12% and none of I shed from the compound after 96 hours. The simulant antigen and antibody reacted on polystyrene microtiter plate and the I was detected by ICP-MS, the detection limit of the method was 0.12 mg·L-1, relative standard deviation (n=9) was less than 3% and the linearly dependent coefficient was 0.998 7. This system can also be used in analysis of other protein, nucleic acid and so on.
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Received: 2009-02-22
Accepted: 2009-05-25
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Corresponding Authors:
WANG Xiao-ru
E-mail: me2elp@fio.org.cn
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[1] Markwell M A K. Analytical Biochemistry，1982，125：427.
[2] HU Ming(胡 明). Chinese Journal of Nuclear Medicine(中华核医学杂志)，1986，6(3)：200.
[3] Goldenberg D M，Goldenberg H，Higginbotham F E，et al. J. Clin. Oncol.，1987，5(11)：1827.
[4] Panyutin I，Neumann R. Acta Oncol.，1996，35：817.
[5] Dewanjee M K, Ghafouripour A K, Kapadvanjwala M, et al. J. Nucl. Med., 1994, 35: 1054.
[6] LIU Bing-chen, YUE Jing-yin, MU Chuan-jie(刘炳辰, 岳井银, 穆传杰). Chinese Journal of Nuclear Medicine(中华核医学杂志), 2000, 20: 33.
[7] Sephen J M, Bruce G W. J. Nucl. Med., 1987, 28: 1034.
[8] Creighton T E. Protein Structure. Oxford: IRL Press, 1989. 288.
[9] QIAO Bin-zong, YANG Yuan, TAO Rui(谯斌宗, 杨 元, 陶 锐). Chinese Journal of Health Laboratory Technology(中国卫生检验杂志), 1998, 8(5): 317.
[10] XIAO Hui-xiang(肖惠祥). Chinese J. Anal. Chem.(分析化学), 1993, 21(11): 1364.
[11] LIU Ren-min, LIU Dao-jie, SUN Xia-ling(柳仁民, 刘道杰, 孙夏玲). Chinese J. Anal. Chem.(分析化学), 1995, 23(4): 407.
[12] GUO Zhong-xian, ZHANG Shu-yun(郭忠先, 张淑云). Analytical Laboratory(分析试验室), 1995, 14(5): 31.
[13] GONG Guo-quan(龚国权). Chinese J. Anal. Chem.(分析化学), 1994, 22(5): 465.
[14] Vanhoe H, Allemeersch F V, Versieck J, et al. Analyst, 1993, 118: 1015.
[15] Larsen E H, Ludwisen M B. J. Anal. At. Spectrom., 1997, 12: 435.
[16] Cox R J, Pickford C J. J. Anal. At. Spectrom., 1992, 7: 635.
[17] Allain P, Mauras Y, Douge C, et al. Analyst, 1990, 115: 813.
[18] Buchert S S A. Fresenius J. Anal. Chem., 1996, 354: 323.
[19] Baumann H. Fresenius J. Anal. Chem., 1990, 338: 809.
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