| 光谱学与光谱分析 |
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| Microdetermination of Proteins with Titan Yellow by the Resonance Light Scattering Method |
| FENG Su-ling, WANG Jin, FAN Jing |
| College of Chemistry and Environmental Science, Key Laboratory of Environmental Science and Engineering of Henan Education Department, Henan Normal University, Xinxiang 453002, China |
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Abstract A resonance light scattering method for the determination of trace proteins was developed. In the presence of Triton X-100, proteins reacted with Titan yellow to form a combination product, resulting a significant enhancement of resonance light scattering(RLS). The ΔIRLS was directly proportional to the concentration of protein in the range of 0.03-0.9 μg·mL-1, with the detection limit 17.7 ng·mL-1 for BSA. This method was applied to the determination of the proteins in synthetic and human serum samples, and compared to the CBB method, with satisfactory results.
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Received: 2003-12-06
Accepted: 2004-03-18
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Corresponding Authors:
FENG Su-ling
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| Cite this article: |
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FENG Su-ling,WANG Jin,FAN Jing. Microdetermination of Proteins with Titan Yellow by the Resonance Light Scattering Method [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2005, 25(06): 927-929.
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| URL: |
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https://www.gpxygpfx.com/EN/Y2005/V25/I06/927 |
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