| 光谱学与光谱分析 |
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| Studies on the Determination of Protein with Spectroscopic Probe of o-Chlorophenylfluorone-Molybdenum(Ⅵ) Complex in Microemulsion Medium |
| DU Bin1, LI Yan1, ZHANG Hui1, WU Dan1, WEI Qin1, LI Zai-jun2 |
1.School of Chemistry and Chemical Engineering, Ji'nan University, Ji'nan 250022, China
2.College of Chemical and Material Engineering, Southern Yangtze University, Wuxi 214036, China |
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Abstract The interaction and absorption spectral behavior of o-chlorophenylfluorone (o-CPF)-Mo(Ⅵ) and protein are studied in OP microemulsion medium.Under the optimum condition, the molar absorptivity of o-CPF-Mo(Ⅵ)-BSA system at 532 nm is 6.12×106 L·mol-1·cm-1, and Beer’s law is obeyed in the range of 0-14 μg·mL-1 of BSA.Moreover, the binding number between the complex and BSA determined by molar ratio and slope ratio methods is 91.The mechanism of interaction of BSA and o-CPF-Mo(Ⅵ) is also discussed.OP microemulsion media introduced into the protein determination increased markedly the sensitivity of the system.The result of experiment indicates that the method possesses a high sensitivity as well as a high selectivity.It can be used to determine protein in human urine with satisfactory results.
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Received: 2004-06-16
Accepted: 2004-09-28
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Corresponding Authors:
DU Bin
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| Cite this article: |
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DU Bin,LI Yan,ZHANG Hui, et al. Studies on the Determination of Protein with Spectroscopic Probe of o-Chlorophenylfluorone-Molybdenum(Ⅵ) Complex in Microemulsion Medium[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2005, 25(07): 1110-1113.
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https://www.gpxygpfx.com/EN/Y2005/V25/I07/1110 |
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