四碘荧光素与牛血清白蛋白相互作用的荧光光谱研究

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Abstract The binding reaction of tetraiodofluorescein with bovine serum albumin in aqueous solution was studied by fluorimetry.The binding constant is 2.65×10⁵ L·mol^-1，the equilibrium constant is 5.7×10⁴ L·mol^-1, and the binding number is 0.86.The research results of binding model indicate that the hydrophobic force was the main binding force.The presence of BSA can increase the fluorescence intensity of TIF.And the ΔF(F-F₀) is linear to the concentration of BSA in a certain range, so the first analytical application was proposed.The linear dynamic range (LDR) was 8×10^-7-9×10^-6 mol·L^-1, the limit of detection(LOD) was 9.88×10^-8 mol·L^-1, and the relative standard deviation(RSD) was 3.4%.

Key words：Tetraiodofluorescein;Bovine serum albumin;Fluorimetry

Received: 2004-09-18 Accepted: 2004-12-28

ZTFLH:

O657.3

Corresponding Authors: DONG Chuan

Cite this article:

LI Gai-xian,LI Jian-qing,WEI Yu-xia, et al. Study on the Interaction of Tetraiodofluorescein and Bovine Serum Albumin by Fluorimetry [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2005, 25(08): 1277-1280.

URL:

https://www.gpxygpfx.com/EN/Y2005/V25/I08/1277

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