| 光谱学与光谱分析 |
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| Study on the Interaction of Sm(Ⅲ) Complexes of Rutin with Serum Albumin |
| WU Jin-xiu1, LI Mei1, SONG Yu-min2, LIU Zhao-gang1, HU Yan-hong1 |
| 1. College of Rare Earth, Inner Mongolia University of Science and Technology, Baotou 014010, China2. College of Chemistry and Chemical Engineering, Northwest Normal University, Lanzhou 730070, China |
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Abstract The binding reaction of rutin-Sm with serum albumin (SA) was investigated by the fluorescence method in physiological condition. The authors studied mainly the quenching mechanism of the fluorescence of SA by rutin-Sm, and calculation of the binding constants KLB of human serum albumin (HSA) and bovine serum albumin(BSA)with rutin-Sm by Lineweaver-Burk equation at different temperatures respectively, then obtained the thermodynamic parameters of HSA and BSA with rutin-Sm according to the calculated binding constants KLB at different temperature, meanwhile the type of binding forces of HSA and BSA with rutin-Sm was determined. The results showed that the emission spectra of BSA(HSA)in the presence and absence of rutin-Sm are different. The emission spectra of BSA(HSA)in the presence of rutin-Sm can be quenched. The quenching mechanism of rutin-Sm to SA was static quenching with non-radiation energy transfer for new complex of SA and rutin-Sm. The binding constants KLB (L·moL-1)were 6.540×105 and 3.265×105 for BSA, and 6.830×105 and 4.665×105 for HSA at 295 K and 310 K respectively. And the type of bonding forces was estimated by the calculation of thermodynamic parameters of the reaction of rutin-Sm with SA at different temperatures, and the result showed that the binding forces were mainly H-bond and Van der Waals between BSA and rutin-Sm due to the ΔH＜0 and ΔS＜0, and the main electrostatic interaction of rutin-Sm and HSA because of ΔH＜0 and ΔS＞0. The effect of rutin-Sm on the conformation of serum albumin was also studied by using synchronous fluorescence spectroscopy. Results indicated that rutin-Sm could be deposited and transported by serum protein in vivo.
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Received: 2008-06-15
Accepted: 2008-09-18
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Corresponding Authors:
LI Mei
E-mail: limei@imust.cn
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