光谱学与光谱分析 |
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Study on the Interaction Mechanism of Antimicrobial Peptide Cecropin-XJ in Xinjiang Silkworm and Staphylococcus Aureus DNA by Spectra |
LIU Zhong-yuan,XU Tao,ZHENG Shu-tao,ZHANG Lan-ting,ZHANG Fu-chun* |
Key Laboratory of Molecule Biology,College of Life Science and Technology,Xinjiang University,Xinjiang Key Laboratory of Biological Resources and Genetic Engineering,Urumqi 830046,China |
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Abstract Nowadays many of antimicrobial peptides have been extensively studied in order to elucidate their antimicrobial mode of action. Much of the research focused on mechanisms of cytoplasmic membrane disruption has been proposed for antimicrobial peptides,but it is not known whether their antimicrobial mode of action is due to their effects on bacterial chromosome. To obtain more information about the possible mechanisms,Cecropin-XJ,a kind of antimicrobial peptide from Xinjiang silkworm,was used as subject and prepared by purification of Pichia yeast fermentation containing cecropin-XJ gene expression product. Subsequently,ultraviolet absorption spectra were employed to investigate whether this antibacterial function is due to Cecropin-XJ works on S. aureus DNA in vitro. The increase in absorbance of DNA samples at 260nm due to the addition of Cecropin-XJ was measured. It is called hyperchromicity of DNA,which can provide a direct measure of the degree of base-pair unstacking. The unstacking results in the loss of duplex helix,and then leads to the duplex helix becoming relaxing. At the same time,the interaction mode was studied by using ethidium bromide (EB) as an extrinsic fluorescence probe. With the addition of Cecropin-XJ,the intensity of intrinsic fluorescence absorbance of DNA at 307 nm increases greatly. It is suggested that the addition of Cecropin-XJ may unstack base-paire of DNA exposing the fluorescent amino acide,leading to the enhancing of DNA fluorescence intensity. Subsequently the competition between Cecropin-XJ and EB to combine with DNA was found. It is suggested that the style was groove binding and intercalation in the interaction of Cecropin-XJ and double helix DNA. Furthermore,in this study,the binding constant and binding number of Cecropin-XJ complex with DNA were determined. There were different binding constant and number of EB complex with DNA because of the the addition of Cecropin-XJ. It is showed that the interaction of Cecropin-XJ and DNA was based on intercalation or non-intercalation. Meanwhile,these results help explain the molecule mechanism of antimicrobial peptide from the interaction style and structure characteristic of Cecropin-XJ and S. aureus DNA. These findings could contribute to further investigation on the mechanism of action of Cecropin-XJ.
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Received: 2007-05-10
Accepted: 2007-08-20
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Corresponding Authors:
ZHANG Fu-chun
E-mail: zfcxju@xju.edu.cn
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[1] Hu Y L,Hu T S,Lin S K,et al. Pharmaceutical Biotechnology,1999,6(4): 193. [2] Andreas R Koczulla,Robert Bals. Drugs,2003,63: 389. [3] WU Dai-fei,ZENG Xian-song,ZHANG Yin-dong,et al(吴代飞,曾宪松,张银东,等). Chinese Journal of Tropical Crops(热带作物学报),1999,20(3):54. [4] LU Xiao-feng,YANG Xing-yong,CHENG Jing-qiu,et al(卢晓风,杨星勇,程惊秋,等). Acta Pharmaceutica Sinica(药学学报),1999,34(2):156. [5] XU Jin-shu,ZHANG Shuang-quan(徐进署,张双全). Acta Entomologica Sinica(昆虫学报),2002,45(5):673. [6] Salditt T,LI Cheng-hao,Spaar A. Biochimica et Biophysica Acta,2006,1758: 1483. [7] Powers J P,Tan A,Ramamoorthy A,et al. Biochemistry,2005,44: 15504. [8] Haukland H H,Ulvatnea H,Sandvika K,et al. FEBS Letters,2001,508(3): 389. [9] Lijuan Zhang,Roland Benz,Robert E W. Biochemistry,1999,38: 8102. [10] WANG Fang,ZHANG Shuang-quan,DAI Zhu-ying(王 芳,张双全,戴祝英). Prog. Biochem. Biophys.(生物化学与生物物理进展),1998,25(1): 64. [11] GONG Xia,SHI Yong-hui,LE Guo-wei(宫 霞,施用晖,乐国伟). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2005,25(3): 420. [12] LIU Zhong-yuan,ZHANG Fu-chun,CAI Lun,et al(刘忠渊,张富春,蔡 伦,等). Acta Microbiologica Sinica(微生物学报),2003,43(5): 635. [13] Ausubel F,Brent R,Robert E K,et al. Short Protocols in Molecular Biology(精编分子生物学实验指南). Translated by YAN Zi-ying,WANG Hai-lin(颜子颖,王海林,译). Beijing: Science Press(北京:科学出版社),1998. 39. [14] SHEN Tong(沈 同著). Biochemistry(生物化学,上册). Beijing: People′s Education Press(北京:人民教育出版社),1989. [15] JIANG Chong-qiu,HE Ji-xiang,WANG Jin-shan(江崇球,贺吉香,王金山). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2002,22(1): 103. [16] LIU Xue-ping,FENG Su-ling,PAN Zi-hong,et al(刘雪平,冯素玲,潘自红,等). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2006,26(10): 1895. [17] Howe G M,Wu K C,Bauer W R. Biochemistry,1976,15(19): 4339. [18] HU Min,ZHANG Zhen-xi,SHEN Guo-li,et al(胡 敏,张镇西,沈国励,等). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2006,26(9): 1668. [19] Kumar C V,Asuncion E H. J. Am. Chem. Soc.,1993,115(19): 8547. [20] Chun Hua Hsu,Chinpan Chen,Maou Lin Jou,et al. Nucleic Acids Research,2005,33(13): 4053. |
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