光谱学与光谱分析 |
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Spatial Orientation Interaction Mechanism of Three Component Complex of Protein ——BSA/SDS/Azur a System |
CAO Wen-gen1, 2, JIAO Qing-cai1,LIU Qian1 |
1. Pharmaceutic Biotechnology Key Laboratory, College of Life Science, Nanjing University, Nanjing 210093, China 2. Department of Biochemistry, Suzhou College, Suzhou 234000, China |
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Abstract The interaction of bovine serum albumin (BSA),sodium dodecyl sulphate (SDS) and Azur A(AA) was studied by spectral probe. The influence of the molar ratio of SDS/BSA on the absorption spectra of BSA-SDS-AA complex was investigated. It was found that the new absorption peak and color changes generated by BSA-SDS-AA complex arose from the difference in the spatial orientation aggregative degree of AA binding on BSA-SDS-AA complex.
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Received: 2003-06-15
Accepted: 2004-11-21
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Corresponding Authors:
CAO Wen-gen
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Cite this article: |
CAO Wen-gen,JIAO Qing-cai,LIU Qian. Spatial Orientation Interaction Mechanism of Three Component Complex of Protein ——BSA/SDS/Azur a System[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2005, 25(09): 1478-1481.
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URL: |
https://www.gpxygpfx.com/EN/Y2005/V25/I09/1478 |
[1] Chial H J, Splittgerber A G. Anal. Biochem., 1993, 213: 362. [2] Congdon R W, Muth G W, Splittgerber A G. Anal. Biochem., 1993, 213: 407. [3] Wei Yong-ju, Li Ke-an, Tong Shen-yang. Talanta, 1996, 43: 1. [4] WEI Yong-ju, LI Ke-an, TONG Shen-yang(魏永巨, 李克安, 童沈阳). Chinese J. of Analytical Chem.(分析化学), 1996, 24(4): 3871. [5] ZHUANG Jia, CHI Yan-hua, LI Ke-an, et al(庄 稼, 迟燕华, 李克安, 等). Acta Chimica Sinica(化学学报), 1998, 56: 827. [6] MA Chun-qi, LI Ke-an, ZHAO Feng-lin, et al(马春琪, 李克安, 赵凤林, 等). Acta Chimica Sinica(化学学报), 1999, 57: 389. [7] LUO Zong-ming, ZHANG Kun, CUI Ying-de, DONG Fei-yan(罗宗铭, 张 琨, 崔英德, 董飞燕). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2001, 21(2): 251. [8] CAO Wen-gen, JIAO Qing-cai, LIU Qian, et al(曹稳根, 焦庆才, 刘 茜, 等). Acta Chimica Sinica(化学学报), 2002, 60(9): 1656. [9] Jiao Qing-cai, Liu Qian. Spectrochimica Acta Part A, 1999, 55: 1667. [10] WAN Hui, OUYANG Wei, JIAO Qing-cai, et al(万 晖, 欧阳炜, 焦庆才, 等). Acta Chimica Sinica(化学学报), 2001, 59: 2181. [11] Liu Qian, Jiao Qing-cai, Liu Zhi-li, et al. Spectroscopy Letters, 2001, 34(1): 25. [12] Zhou Shang-gen, Jiao Qing-cai, CHEN Lei, et al. Spectroscopy Letters, 2002, 35(1): 21. [13] Cao Wen-gen, Jiao Qing-cai, Fu Yu, et al. Spectroscopy Letters, 2003, 36(3): 197. [14] LI Chao-long, CHEN Shu-qing, LIU Zi-yi(厉朝龙, 陈枢青, 刘子贻). The Experimental Technology of Biochemistry and Molecular Biology(生物化学与分子生物学实验技术). Hangzhou: Hangzhou University Press(杭州: 浙江大学出版社), 2000. 72. [15] Friedenauer S, Berlet H H. Anal. Biochem., 1989, 178: 263. [16] Lea M A, Grasso S V, Hu J, et al. Anal. Biochem., 1984, 141: 390. [17] CAO Wen-gen, CHEN Lei, JIAO Qing-cai, DING Li-hua(曹稳根, 陈 雷, 焦庆才, 丁黎华). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2003, 23(3): 587.
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