光谱学与光谱分析 |
|
|
|
|
|
Thermodynamics Studies on the Binding of Rutin and Serum Albumin |
LUAN Ni-na1,WU Jin-xiu1,2,SONG Yu-min1*,WU Qiong1 |
1. College of Chemistry and Chemical Engineering, Northwest Normal University, Lanzhou 730070, China2. College of Rare Earth, Inner Mongolia University of Science and Technology, Baotou 014010, China |
|
|
Abstract To study the binding of rutin and serum albumin (SA) in physiological condition and the quenching mechanism of the fluorescence of SA by rutin, the fluorescence method was used. The results shows that the emission spectra of BSA(HSA)in the presence and absence of rutin are different. The emission spectra of BSA(HSA)in the presence of rutin can be quenched. The quenching mechanism of rutin to SA was static quenching with non-radiation energy transfer with single molecule. The binding constants KA, the number of binding sites n and the thermodynamic parameters of the reaction of rutin with SA were determined at different temperatures. At 295 and 310 K, for BSA, KA(L·moL-1)=4.215×104 and 6.996×103 and n=0.75 and 0.64, respectively; for HSA,KA(L·moL-1)=2.660×104 and 4.110×103 and n=0.70 and 0.60, respectively. The binding constants KA decreased with the increase in temperature, which means that rutin and SA have a quite strong ability to form a new complex-system. The main binding force was discussed by thermodynamic equation, and the result is that ΔH<0 and ΔS<0 for the reaction of rutin with SA. So the binding forces was mainly H-bond and Van der Waals. The effect of the drug on the conformation of serum albumin was also studied by using synchronous fluorescence spectroscopy. Rutin could be deposited and transported by serum protein in vivo. Rutin had nearly no effect on the serum protein conformation.
|
Received: 2007-02-06
Accepted: 2007-05-09
|
|
Corresponding Authors:
SONG Yu-min
E-mail: peterxjp@126.com
|
|
[1] LIU Chang-xiao(刘昌孝). Acta Pharm. Sin.(药学学报), 2005,40: 395. [2] LAN Chang-yun, ZHOU Chong-song, FAN Bi-wei, et al(兰昌云, 周崇松, 范必威, 等). Natural Product Research and Development(天然产物研究与开发),2005,17: 55. [3] Jingsu New Medicine College(江苏新药学院编). Dictionary of Chinese Materia Mediaca(中药大词典). Shanghai: Shanghai Scientific and Technical Publishers(上海: 上海科学技术出版社), 1977. 2433. [4] Ulrich K H. Pharmacological Review, 1981, 33: 17. [5] CAO Shu-xia, ZHAO Yu-fen(曹书霞, 赵玉芬). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2004, 24(10): 1197. [6] XU Wen-xiang, PANG Yue-hong, SHUANG Shao-min(徐文祥, 庞月红, 双少敏). Chinese Journal of Analytical Chemistry(分析化学), 2004, 32(12): 1571. [7] Rost B. Structure, 1998, 6(3): 259. [8] WU Gen-hua, WANG Chun-hua(吴根华, 汪春华). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2005, 25(2): 246. [9] YU Tian-zhi, YANG Ru-dong(愈天智, 扬汝栋). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2003, 23(4): 763. [10] CHEN Guo-zhen, HUANG Xian-zhi, ZHENG Zhu-zi, et al(陈国珍,黄贤智,郑朱梓,等). Fluorescence Analytical Method(荧光分析法). Beijing: Science Press(北京: 科学出版社), 1990. 122. [11] YI Ping-gui, SHANG Zhi-cai, YU Qing-sen(易平贵, 商志才, 愈庆森). Acta Chim. Sin.(化学学报), 2000, 58: 1649. [12] YANG Mei-ling, YANG Pei-ju, SONG Yu-min(杨美玲, 杨培菊, 宋玉民). Chinese Journal of Inorganic Chemistry(无机化学学报), 2005, 21(4): 483. [13] SONG Yu-min, WU Jin-xiu, ZHENG Xiu-rong(宋玉民, 吴锦绣, 郑秀荣). Chinese Journal of Inorganic Chemistry(无机化学学报), 2006, 22(9): 1615. [14] LIU Xiu-ping, ZHANG Guo-mei, YANG Yu, et al(刘秀萍,张国梅,杨 郁,等). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2003,23(4):323. [15] Lo Kenneth Kam-Wing, Hui Wai-Ki, Chung Chi-Keung. Coordination Chemistry Reviews, 2005, 249(13-14): 1434. [16] Malgorzata Jezowska-Bojczuk, Henryk Kozlowski, Kalalin Varnagy, et al. J. Inorg. Biochem., 1990,40:357. [17] CHEN Xiao-bo, LI Song(陈晓波, 李 崧). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2006,26(2):309. [18] Ross P D, Subramanian S. Biochemistry, 1981, 20: 3096. [19] Gonzalez-Jzmenoz J, Jacquotte H, Cayre I. Chemico-Biological Interactions, 1992, 84: 221. [20] SONG Yu-min, WU Jin-xiu(宋玉民, 吴锦绣). Chinese Journal of Inorganic Chemistry(无机化学学报), 2006, 22(12): 2165. [21] SHENG Liang-quan, YAN Xiang-yang, XU Hua-jie, et al(盛良全, 闫向阳, 徐华杰,等). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2007,27(2):306. |
[1] |
TAN Ai-ling1, WANG Si-yuan1, ZHAO Yong2, ZHOU Kun-peng1, LU Zhang-jian1. Research on Vinegar Brand Traceability Based on Three-Dimensional Fluorescence Spectra and Quaternion Principal Component Analysis[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(07): 2163-2169. |
[2] |
ZHOU Meng-ran1, LAI Wen-hao1*, WANG Ya1, 2, HU Feng1, LI Da-tong1, WANG Rui1. Application of CNN in LIF Fluorescence Spectrum Image Recognition of Mine Water Inrush[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(07): 2262-2266. |
[3] |
CHEN Ji-wen1, XU Tao2, LIU Wei2, FANG Zhe1, QU Hua-yang1*, LIANG Yuan1, HU Xue-qiang1, LIU Ming-bo1. On-Line Determination of Light-Rare Earth Distribution by Energy Dispersive-X-Ray Fluorescence[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(07): 2284-2289. |
[4] |
GONG Han-qing, CHEN Jian-bo*. The Interaction between 4-Ethyl-2-Methoxyphenol and Human Serum Albumin Studied by Spectroscopic and Molecular Docking Techniques[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(06): 1869-1873. |
[5] |
LIU Ling1, YANG Ming-xing1, 2*, LU Ren1, Andy Shen1, HE Chong2. Study on EDXRF Method of Turquoise Composition[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(06): 1910-1916. |
[6] |
ZHANG Li-jiao1,2, LAI Wan-chang1, XIE Bo2, 3, HUANG Jin-chu1, LI Dan1, WANG Guang-xi1, YANG Qiang1, CHEN Xiao-li1. The Effect of Filterson on the Determination of Trace Heavy Metal Cd in Light Matrix by Energy Dispersive X-Ray Fluorescence Spectrometry[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(06): 1917-1921. |
[7] |
TANG Zhu-rui1, 2, XI Bei-dou1, 3, 4, HE Xiao-song1, 3, TAN Wen-bing1, 3, ZHANG Hui1, 3, LI Dan1, 3, HUANG Cai-hong1, 3*. Structural Characteristics of Dissolved Organic Compounds during Swine Manure Composting[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(05): 1526-1532. |
[8] |
ZHOU Meng-ran, HU Feng*, YAN Peng-cheng, LIU Dong. Laser Induced Fluorescence Spectrum Analysis of Water Inrush in Coal Mine Based on FCM[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(05): 1572-1576. |
[9] |
WANG Shi-fang, LUO Na, HAN Ping*. Application of Energy-Dispersive X-Ray Fluorescence Spectrometry to the Determination of As, Zn,Pb and Cr in Soil[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(05): 1648-1654. |
[10] |
ZHANG Qiu-hui1, GUO Zhuang-zhi1, FENG Guo-ying2. The Effect of Incident Laser Power on Raman Spectra and Photoluminescence Spectra of Silicon Nanowires[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(04): 1118-1121. |
[11] |
LI Shuang-fang1,2, GUO Yu-bao1*, SUN Yan-hui2, GU Hai-yang2. Rapid Identification of Sunflower Seed Oil Quality by Three-Dimensional Synchronous Fluorescence Spectrometry[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(04): 1165-1170. |
[12] |
ZHU Cong-hai1, 3, CHEN Guo-qing1, 3*, ZHU Chun1, 2, 3, ZHAO Jin-chen1, 3, LIU Huai-bo1, 3, ZHANG Xiao-he1, 3, SONG Xin-shu1, 3. Studies of the Fluorescence Properties of Methanol and Ethanol Based on the Density Functional Theory[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(04): 1133-1138. |
[13] |
OUYANG Heng1,2*, XIAO Jian-ren3, LIN Xiu-yong4, FAN Gong-duan4*. Compositional Characteristics of Dissolved Organic Matter in Water Treatment Systems of Water Source Heat Pump Based on Three-Dimensional Fluorescence Analysis[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(04): 1146-1152. |
[14] |
WANG Yu-tian, LIU Ting-ting*, LIU Ling-fei, YANG Zhe, CUI Yao-yao. Determination of Polycyclic Aromatic Hydrocarbons in Water Based on Three Dimensional Fluorescence Spectroscopy Combined with Wavelet Compression and APTLD[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(04): 1171-1177. |
[15] |
TANG Dong-lin1, WANG Qiao1, CHU Yi-neng2, LI Rui-hai2. Detecting H2S Gas Concentration by 1,8-Naphthalimides Fluorescent Probe[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(04): 1319-1323. |
|
|
|
|