| 光谱学与光谱分析 |
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| Interaction of Surface Active Fluorescence Probes and Bovine Serum Albumin |
| XU Tong-kuan, LI Na, SHEN Xing-hai*, GAO Hong-cheng |
| Department of Applied Chemistry, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China |
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Abstract The binding between two surface-active substituted 3H-indole fluorescence probes, i.e., iodo-dihexadecyl methyl-2-(p-dodecyl amino phenyl)-3, 3-dimethyl-5-carboethoxy-3H-indole ammonium and iodo-dimethyloctadecyl-2-(p-dodecyl amino phenyl)-3,3-dimethyl-5-carboethoxy-3H-indole ammonium, and bovine serum albumin (BSA) in aqueous solution was studied using fluorescence. The binding constant and binding site number of molecule 1 and molecule 2 with BSA were obtained. It was confirmed that electrostatic interaction is the primary driving force for the combination of BSA with molecule 1 or molecule 2. According to the Frster resonance energy transfer theory, the distances between molecule 1, molecule 2 and tryptophan of BSA were calculated to be 2.90 nm and 4.02 nm, respectively.
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Received: 2004-03-01
Accepted: 2004-06-06
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Corresponding Authors:
SHEN Xing-hai
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| Cite this article: |
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XU Tong-kuan,LI Na,SHEN Xing-hai, et al. Interaction of Surface Active Fluorescence Probes and Bovine Serum Albumin[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2005, 25(09): 1443-1445.
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| URL: |
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https://www.gpxygpfx.com/EN/Y2005/V25/I09/1443 |
[1] Deep S, Ahluwalia J C. Phys. Chem. Chem. Phys., 2001, 3：4583.
[2] LI Jian, SHEN Xing-hai, GAO Hong-cheng. Chem. Phys. Lett., 2001, 342: 529.
[3] XU Tong-kuan, SHEN Xing-hai, GAO Hong-cheng(徐同宽，沈兴海，高宏成). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2005, 25(6): 857.
[4] XU Tong-kuan, SHEN Xing-hai, GAO Hong-cheng(徐同宽，沈兴海，高宏成). Chin. Chem. Lett., 2004, 15：26.
[5] Philip Ross D, Sabramanian S. Biochemistry, 1981, 20: 3096.
[6] LIU Bao-sheng, ZHANG Hong-yi, WANG Fu-li(刘保生，张红医，王甫丽). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2003, 23(3): 539.
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