| 光谱学与光谱分析 |
|
|
|
|
|
| Interaction of Surface Active Fluorescence Probes and Bovine Serum Albumin |
| XU Tong-kuan, LI Na, SHEN Xing-hai*, GAO Hong-cheng |
| Department of Applied Chemistry, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China |
|
|
|
|
Abstract The binding between two surface-active substituted 3H-indole fluorescence probes, i.e., iodo-dihexadecyl methyl-2-(p-dodecyl amino phenyl)-3, 3-dimethyl-5-carboethoxy-3H-indole ammonium and iodo-dimethyloctadecyl-2-(p-dodecyl amino phenyl)-3,3-dimethyl-5-carboethoxy-3H-indole ammonium, and bovine serum albumin (BSA) in aqueous solution was studied using fluorescence. The binding constant and binding site number of molecule 1 and molecule 2 with BSA were obtained. It was confirmed that electrostatic interaction is the primary driving force for the combination of BSA with molecule 1 or molecule 2. According to the Frster resonance energy transfer theory, the distances between molecule 1, molecule 2 and tryptophan of BSA were calculated to be 2.90 nm and 4.02 nm, respectively.
|
|
Received: 2004-03-01
Accepted: 2004-06-06
|
|
|
|
Corresponding Authors:
SHEN Xing-hai
|
|
| Cite this article: |
|
XU Tong-kuan,LI Na,SHEN Xing-hai, et al. Interaction of Surface Active Fluorescence Probes and Bovine Serum Albumin[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2005, 25(09): 1443-1445.
|
|
|
|
| URL: |
|
http://www.gpxygpfx.com/EN/Y2005/V25/I09/1443 |
[1] Deep S, Ahluwalia J C. Phys. Chem. Chem. Phys., 2001, 3：4583.
[2] LI Jian, SHEN Xing-hai, GAO Hong-cheng. Chem. Phys. Lett., 2001, 342: 529.
[3] XU Tong-kuan, SHEN Xing-hai, GAO Hong-cheng(徐同宽，沈兴海，高宏成). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2005, 25(6): 857.
[4] XU Tong-kuan, SHEN Xing-hai, GAO Hong-cheng(徐同宽，沈兴海，高宏成). Chin. Chem. Lett., 2004, 15：26.
[5] Philip Ross D, Sabramanian S. Biochemistry, 1981, 20: 3096.
[6] LIU Bao-sheng, ZHANG Hong-yi, WANG Fu-li(刘保生，张红医，王甫丽). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2003, 23(3): 539.
|
| [1] |
ZHANG Xiu-qing, PENG Jun, LING Jian*, LIU Chao-juan, CAO Qiu-e, DING Zhong-tao . Fluorescence Resonance Energy Transfer Detection of Cobalt Ions by Silver Triangular Nanoplates and Rhodamine 6G[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2015, 35(04): 951-955. |
| [2] |
WANG Xiao-mei1, YE Chao1, LI Chen-gui1, HU Yu-zhu1, 2* . Study on the Fluorescence Resonance Energy Transfer between CdTe QDs and Rhodamine B and Its Application to the Determination of Trace Amount of Copper in Flos Lonicerae Japonicae[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2011, 31(02): 448-451. |
| [3] |
DENG Chu-yun, LI Jia-min, MA Wan-yun*. DNA Nanosensor Fluorescence Imaging Microscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2010, 30(01): 220-224. |
| [4] |
LI Jin-gui1, ZHU Kui2, XU Fei2, JIANG Hai-yang2, DING Shuang-yang2* . Fluorescence Resonance Energy Transfer between Gentamycin and Water-Soluble CdTe QDs[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2009, 29(11): 3070-3074. |
| [5] |
CHEN Tong-sheng1,WANG Xiao-ping2,SUN Lei1,WANG Hui-ying1,WANG Long-xiang1. Fluorescence Analysis of Taxol-Induced Paraptosis-Like Independent of Caspase-3 Activation[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2008, 28(11): 2623-2627. |
| [6] |
CHEN Tong-sheng1,WANG Xiao-ping2,WANG Zhi-ping3,WANG Long-xiang1,WANG Hui-ying1,XING Da1. Fluorescence Emission Analysis of Caspase-3 Activation Induced by Xiao-Ai-Ping (XAP) inside Living Human Lung Adenocarcinoma Cells[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2008, 28(06): 1327-1331. |
| [7] |
MI Xian-wen1,3,WANG Yong-sheng1,2*,XUE Jin-hua1,OUYANG Yun-fu1,YANG Sheng-yuan1,WANG Ying1 . Determination of 8-Hydroxy-2’-Deoxyguanosine in Urine with Protamine Using Resonance Energy Transfer Fluorescence Quenching by Sensitizing Effect of Triton X-100 [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2007, 27(08): 1570-1573. |
| [8] |
CHEN Dan-dan1,ZHANG Tao1,REN Li-ping1*,SHI Deng-rong2,YU Jin-lian2 . Study on the Spectra of Cal-Red-Cu(Ⅱ) Complex with Bovine Serum Albumin and Its Analytical Application[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2006, 26(04): 686-689. |
| [9] |
LIU Bao-sheng, GAO Jing, LIU Zhi-chao, YU Li-na, YANG Da-cheng, YANG Geng-liang. Fluorescence Resonance Energy Transfer Quenching Method for Determination of Arsenic with Acridine Orange-Rhodamine B[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2006, 26(02): 306-308. |
| [10] |
XU Tong-kuan, SHEN Xing-hai*, GAO Hong-cheng. Synthesis of Substituted 3H-Indole Quaternary Ammonium Salt and Study on Its Interaction with Bovine Serum Albumin by Fluorescence [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2005, 25(06): 857-860. |
| [11] |
XIAO Hou-rong, SHENG Liang-quan, SHI Chun-hua, XU Xiao-long, XIE Yong-shu, LIU Qing-liang . Fluorescence Study on the Interaction of Salicylic Acid and Bovine Serum Albumin [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2004, 24(01): 78-81. |
|
|
|
|
