光谱学与光谱分析 |
|
|
|
|
|
Studies on Thermal Denaturation of Peanut Allergen Ara h1 and its Interaction with Reducing Sugars |
XU Hong1, SHEN Liang-liang1, HU Zhang-li1*, XIAO Jie3*, XIAO Hua-xin2, WU Jin-xia2, LI Yi1, WEI Bo3, NI Zhuo3, WU Xu-li4, LIU Zhi-gang4 |
1. College of Life Sciences, Shenzhen Key Laboratory of Marine Bioresources and Ecology, Shenzhen University, Shenzhen 518060, China 2. College of Life Sciences, Shenzhen Key Laboratory of Microbial Genetic Engineering, Shenzhen 518060, China 3. College of Chemistry and Chemical Engineering, Shenzhen University, Shenzhen 518060, China 4. College of Medical Science, Shenzhen University, Shenzhen 518060, China |
|
|
Abstract The thermal denaturation of peanut allergen Ara h1, its interaction with reducing sugars and the corresponding changes in allergenicity were investigated by circular dichroism(CD), fluorescence and ELISA method comprehensively. The experimental results indicate that the secondary structure of Ara h1 changes significantly along with decreasing α-helical structure and its allergenicity with the temperature higher than 85 ℃, and that both xylose and fructose can stabilize Ara h1 protein structure through interacting with Ara h1 protein and decrease its allergenicity obviously. This study should be helpful to the further understanding of sensitization mechanism of food allergy and be useful for the guidance on reasonable manufacturing of peanut foods.
|
Received: 2012-11-30
Accepted: 2013-03-21
|
|
Corresponding Authors:
HU Zhang-li, XIAO Jie
E-mail: huzl@szu.edu.cn; chinafujian_pt@163.com
|
|
[1] Viquez O M, Konan K N. The Journal of Allergy and Clinical Immunology, 2002, 109(1): 48. [2] Wijk F V, Hartgring S, Koppelman S J. Clinical and Experimental Allergy, 2004, 34(9): 1422. [3] Barre A, Borges J P, Culerrier R, et al. Immunology Letters, 2005, 100(2): 153. [4] Lehmann K, Hoffmann K, Neudecker P, et al. Protein Expression and Purification, 2003, 31(2): 250. [5] Barre A, Borges J P, Rouge P, et al. Immunology Letters, 2005, 100(2): 153. [6] Leon L P D, Drew A D, Glaspole I N, et al. Molecular Immunology, 2007, 44(4): 463. [7] Kang I H, Gallo M. Plant Science, 2007, 172(2): 345. [8] Viquez O M, Konan K N, Hortense, et al. Molecular Immunology, 2004, 41(12): 1235. [9] HUANG Han-chang, JIANG Zhao-feng, ZHU Hong-ji(黄汉昌,姜招峰,朱宏吉). Chemistry Bulletin(化学通报), 2007, 70(7): 501. [10] DING Xiao-lan, GAO Hong-qi(丁晓岚,高红旗). Experimental Technology and Management(实验技术与管理), 2008, 25(10): 48. [11] Sceerama N, et al. Analytical Biochemistry, 2000, 287(2): 243. [12] SHEN Xing-can, LIANG Hong, HE Xi-wen, et al(沈星灿,梁 宏,何锡文,等). Chinese Journal of Analytical Chemistry(分析化学), 2004, 32(3): 388. [13] GUO Yao-jun(郭尧君). Spectrophotometry and Its Applicationin in Biochemistry(分光光度技术及其在生物化学中的应用). Beijing: Science Press(北京:科学出版社), 1987. 230. [14] Timasheff S N. Annual Review of Biophysics and Biomolecular Structure, 1993, 22(1): 67. [15] Stef J K, Carla A, Bruijnzeel-Koomen F M, et al. Biological Chemistry, 1999, 274(1): 4770. |
[1] |
LEI Hong-jun1, YANG Guang1, PAN Hong-wei1*, WANG Yi-fei1, YI Jun2, WANG Ke-ke2, WANG Guo-hao2, TONG Wen-bin1, SHI Li-li1. Influence of Hydrochemical Ions on Three-Dimensional Fluorescence
Spectrum of Dissolved Organic Matter in the Water Environment
and the Proposed Classification Pretreatment Method[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 134-140. |
[2] |
XIA Ming-ming1, 2, LIU Jia3, WU Meng1, 2, FAN Jian-bo1, 2, LIU Xiao-li1, 2, CHEN Ling1, 2, MA Xin-ling1, 2, LI Zhong-pei1, 2, LIU Ming1, 2*. Three Dimensional Fluorescence Characteristics of Soluble Organic Matter From Different Straw Decomposition Products Treated With Calcium Containing Additives[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 118-124. |
[3] |
GU Yi-lu1, 2,PEI Jing-cheng1, 2*,ZHANG Yu-hui1, 2,YIN Xi-yan1, 2,YU Min-da1, 2, LAI Xiao-jing1, 2. Gemological and Spectral Characterization of Yellowish Green Apatite From Mexico[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 181-187. |
[4] |
HAN Xue1, 2, LIU Hai1, 2, LIU Jia-wei3, WU Ming-kai1, 2*. Rapid Identification of Inorganic Elements in Understory Soils in
Different Regions of Guizhou Province by X-Ray
Fluorescence Spectrometry[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 225-229. |
[5] |
LIU Wei1, 2, ZHANG Peng-yu1, 2, WU Na1, 2. The Spectroscopic Analysis of Corrosion Products on Gold-Painted Copper-Based Bodhisattva (Guanyin) in Half Lotus Position From National Museum of China[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3832-3839. |
[6] |
WANG Hong-jian1, YU Hai-ye1, GAO Shan-yun1, LI Jin-quan1, LIU Guo-hong1, YU Yue1, LI Xiao-kai1, ZHANG Lei1, ZHANG Xin1, LU Ri-feng2, SUI Yuan-yuan1*. A Model for Predicting Early Spot Disease of Maize Based on Fluorescence Spectral Analysis[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3710-3718. |
[7] |
CHENG Hui-zhu1, 2, YANG Wan-qi1, 2, LI Fu-sheng1, 2*, MA Qian1, 2, ZHAO Yan-chun1, 2. Genetic Algorithm Optimized BP Neural Network for Quantitative
Analysis of Soil Heavy Metals in XRF[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3742-3746. |
[8] |
SONG Yi-ming1, 2, SHEN Jian1, 2, LIU Chuan-yang1, 2, XIONG Qiu-ran1, 2, CHENG Cheng1, 2, CHAI Yi-di2, WANG Shi-feng2,WU Jing1, 2*. Fluorescence Quantum Yield and Fluorescence Lifetime of Indole, 3-Methylindole and L-Tryptophan[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3758-3762. |
[9] |
WANG Zhi-qiang1, CHENG Yan-xin1, ZHANG Rui-ting1, MA Lin1, GAO Peng1, LIN Ke1, 2*. Rapid Detection and Analysis of Chinese Liquor Quality by Raman
Spectroscopy Combined With Fluorescence Background[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3770-3774. |
[10] |
YI Min-na1, 2, 3, CAO Hui-min1, 2, 3*, LI Shuang-na-si1, 2, 3, ZHANG Zhu-shan-ying1, 2, 3, ZHU Chun-nan1, 2, 3. A Novel Dual Emission Carbon Point Ratio Fluorescent Probe for Rapid Detection of Lead Ions[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3788-3793. |
[11] |
YANG Ke-li1, 2, PENG Jiao-yu1, 2, DONG Ya-ping1, 2*, LIU Xin1, 2, LI Wu1, 3, LIU Hai-ning1, 3. Spectroscopic Characterization of Dissolved Organic Matter Isolated From Solar Pond[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3775-3780. |
[12] |
QI Guo-min1, TONG Shi-qian1, LIN Xu-cong1, 2*. Specific Identification of Microcystin-LR by Aptamer-Functionalized Magnetic Nanoprobe With Laser-Induced Fluorescence[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3813-3819. |
[13] |
HE Yan-ping, WANG Xin, LI Hao-yang, LI Dong, CHEN Jin-quan, XU Jian-hua*. Room Temperature Synthesis of Polychromatic Tunable Luminescent Carbon Dots and Its Application in Sensitive Detection of Hemoglobin[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(11): 3365-3371. |
[14] |
LIN Hong-jian1, ZHAI Juan1*, LAI Wan-chang1, ZENG Chen-hao1, 2, ZHAO Zi-qi1, SHI Jie1, ZHOU Jin-ge1. Determination of Mn, Co, Ni in Ternary Cathode Materials With
Homologous Correction EDXRF Analysis[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(11): 3436-3444. |
[15] |
LI Xiao-li1, WANG Yi-min2*, DENG Sai-wen2, WANG Yi-ya2, LI Song2, BAI Jin-feng1. Application of X-Ray Fluorescence Spectrometry in Geological and
Mineral Analysis for 60 Years[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(10): 2989-2998. |
|
|
|
|