| 光谱学与光谱分析 |
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| Effect of Heat Treatment on the Antigenicity and Conformation of Peanut Allergen Ara h 2 |
| HU Chun-qiu1, 2, GAO Jin-yan1, 3, CHEN Hong-bing1, 2*, LUO Chun-ping1, 2, YAN Fei1, 2 |
1. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China
2. Sino-German Joint Research Institute, Nanchang University, Nanchang 330047, China
3. Department of Food Science, Nanchang University, Nanchang 330047, China |
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Abstract Peanut allergen Ara h 2 was extracted from peanuts and was identified by SDS-PAGE and MALDI-TOF-MS. Effect of heat treatment on the antigenicity and structure of Ara h 2 was measured by indirect ELISA, CD, fluorescence and UV absorption spectra. The results showed that the antigenicity of Ara h 2 had a slight increase after being heated at 55 or 70 ℃, while above 85 ℃ the antigenicity decreased significantly, and the antigeicity of Ara h 2 decreased with increasing temperature. The CD showed that the secondary structure of Ara h 2 was changed after heat treatment. The ANS fluorescence probe emission spectra analysis demonstrated that the heat treatment induced an increase in surface hydrophobicity of Ara h 2. The UV absorption spectra showed that the absorption maximum wavelength was increased when Ara h 2 was heated except the sample heating at 50 ℃ for 30 min. So the changes in conformation of Ara h 2 lead to the antigenicity degression.
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Received: 2010-02-10
Accepted: 2010-05-20
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Corresponding Authors:
HU Chun-qiu
E-mail: chbgjy@hotmail.com
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