| 光谱学与光谱分析 |
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| Study on the Spectroscopy and Electrochemistry of the Combination between Samarium(Ⅲ) Ion Probe and Bovine Serum Albumin |
| LI Lei, LI Xun, QI Xun, LUO Guo-tian |
| Department of Chemistry and Life Science, Gannan Teacher's College, Ganzhou 341000, China |
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Abstract The synthesis of solid compound of bovine serum albumin (BSA) with samarium (Ⅲ) ion was studied. The FTIR spectrum shows that samarium (Ⅲ) ion forms high sensitivity compound with BSA′s oxygen in hydroxyl and nitrogen in amidocyanogen or amide groups. The ultraviolet spectrum shows that samarium (Ⅲ) ion binds directly with residue of tyrosine. The character of the combination between samarium (Ⅲ) ion and BSA was studied under simulated physiology conditions. The fluorescence spectrum shows that the molar ratio of samarium (Ⅲ) ion to BSA is 2.5∶1, with apparent complex constant lgK=11.00. By cyclic voltammetry, the paper reports the stability of compound of BSA with samarium (Ⅲ) ion and the effect pH on the of combination between samarium (Ⅲ) ion and BSA.
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Received: 2002-10-21
Accepted: 2003-01-28
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Corresponding Authors:
LI Lei
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| Cite this article: |
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LI Lei,LI Xun,QI Xun, et al. Study on the Spectroscopy and Electrochemistry of the Combination between Samarium(Ⅲ) Ion Probe and Bovine Serum Albumin [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2004, 24(01): 74-77.
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| URL: |
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http://www.gpxygpfx.com/EN/Y2004/V24/I01/74 |
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