Research on Fluorescence Spectral Characteristics of Phycocyanin under Different Habitat Conditions
ZHANG Xiao-ling1,2,3, YIN Gao-fang1,2,3, ZHAO Nan-jing1,2,3*, QIN Zhi-song1,2,3, XIAO Xue1,2,3, DUAN Jing-bo1,2,3, YANG Rui-fang1,2,3, TU Meng-di1,2,3, LIU Jian-guo1,2,3, LIU Wen-qing1,2,3
1. Key Laboratory of Environmental Optics and Technology, Anhui Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Hefei 230031, China
2. University of Science and Technology of China, Hefei 230036, China
3. Key Laboratory of Optical Monitoring Technology for Environment, Anhui Province, Hefei 230031, China
Abstract：The development of cyanobacterial biomass detection technology is an important part to deal with the frequent occurrence of water bloom events. Phycocyanin, to some extent, as a special protein of cyanobacteria, is more accurate reaction in natural water cyanobacterial biomassthan chlorophyll, thus become an important index in cyanobacterial biomass detection. This paper, with different light intensity and different growth stages as the research objects. compares the reliability of the two methods of the envelope method and the single point method , exploring the fluorescence spectral characteristics of phycocyanin in vivo Microcystis Anabaena by using three-dimensional fluorescence spectroscopy. The results show that：(1) The fluorescence intensity increases with the growth of long-term; (2) It is more reliable to use envelope method to analyze the characteristic fluorescence spectra of the fluorescent spectrum than the single point method. (3) The fluorescence spectra of Phycocyanin in vivo Microcystis were basically unchanged in EX=614 nm/ EM=654 nm. The excitation wavelength of the phycocyanin fluorescence in vivo Microcystis decreases with the growth with 610 and 620 nm in the long term, and the emission wavelength varied between 650 and 660 nm, which is related to the sample particle size and spectral scanning mode. This study provided the experimental basis for the development of the fluorescent detection technology of cyanobacteria.
Key words：Phycocyanin; In vivo fluorescence; Cyanobacteria; Phytoplankton
张小玲，殷高方，赵南京，覃志松，肖 雪，段静波，杨瑞芳，涂梦迪，刘建国，刘文清. 不同生境条件下藻蓝蛋白活体荧光光谱特性研究[J]. 光谱学与光谱分析, 2017, 37(04): 1145-1151.
ZHANG Xiao-ling, YIN Gao-fang, ZHAO Nan-jing, QIN Zhi-song, XIAO Xue, DUAN Jing-bo, YANG Rui-fang, TU Meng-di, LIU Jian-guo, LIU Wen-qing. Research on Fluorescence Spectral Characteristics of Phycocyanin under Different Habitat Conditions. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2017, 37(04): 1145-1151.
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