Spectral Changes of C-Phycocyanin with Different Molar Ratios of SPDP
YAN Shi-gan1,3,CHEN Xiu-lan1,ZHANG Xi-ying1,ZHOU Bai-cheng1,2,ZHANG Yu-zhong1*
1. State Key Lab of Microbial Technology, Shandong University, Ji’nan 250100, China 2. Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China 3. Shandong Key Lab of Animal Disease Control and Breeding, Ji’nan 250100, China
Abstract：Pure C-phycocyanin was prepared from Spirulina platensis using one-step anion-exchange chromatography. The C-PC obtained was with an absorption maximum at 620 nm and a fluorescence emission maximum at 640 nm when excited by 580 nm. SPDP is an excellent heterobifunctional crosslinker for thiolating amines. Different molar ratios of SPDP have remarkable influence on the absorption and fluorescence spectra of C-phycocyanin. The absorption maximum and fluorescence emission maximum both decreased and blue-shifted from 640 nm to 630 nm as the molar ratios of SPDP increased. It was found that the molar ratios of SPDP to C-phycocyanin was not more than 100 was appropriate to being conjugated with other biomolecules from the absorption and fluorescence spectra of C-phycocyanin.
颜世敢1,3,陈秀兰1,张熙颖1,周百成1,2,张玉忠1*. 异型双功能交联剂SPDP对C-藻蓝蛋白的光谱影响[J]. 光谱学与光谱分析, 2008, 28(05): 1115-1117.
YAN Shi-gan1,3,CHEN Xiu-lan1,ZHANG Xi-ying1,ZHOU Bai-cheng1,2,ZHANG Yu-zhong1*. Spectral Changes of C-Phycocyanin with Different Molar Ratios of SPDP. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2008, 28(05): 1115-1117.
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