Effect of Soil Moisture Content on the Fluorescence Characteristics of Polycyclic Aromatic Hydrocarbons
杨仁杰1, 孙雪杉1, 王 斌2，董桂梅1, 杨延荣1，周长宏1，张伟玉1*，刘海学3*
1. College of Engineering and Technology, Tianjin Agricultural University, Tianjin 300384, China
2. The Photonics Center of The Physics Institute, Nankai University, Tianjin 300071, China
3. Laboratory of Agricultural Analysis，Tianjin Agricultural University, Tianjin 300384, China
Abstract：Fluorescence spectroscopy has been used to detect polycyclic aromatic hydrocarbons (PAHs) in soil. However, the soil moisture has a strong interference to the fluorescence intensity of PAHs, which is a challenge to the development of the rapid real-time detection technology of PAHs in soil. In order to analyze the effect of soil moisture on the fluorescence characteristics of PAHs, 8 phenanthrene contaminated soil samples were prepared with different moisture content (5%~40%, interval of 5%). The dynamic one-dimensional fluorescence spectra of soil samples with different moisture content were obtained by LS-55 fluorophotometer. Two-dimensional (2D) correlation fluorescence spectrum was calculated under the perturbation of soil moisture content. It was found that the fluorescence intensity of phenanthrene at 386, 408 and 432 nm enhanced with the increase of soil moisture content, while the Rayleigh scattering light intensity at 333 nm was decreased. And, it is pointed out that the effect of soil moisture on fluorescence can be corrected by establishing the relationship among fluorescence intensity, Rayleigh scattering intensity and soil moisture content. Meanwhile, the effect of soil moisture on the standard curve of phenanthrene concentration was studied. It is demonstrated that the soil moisture has a great influence on the quantitation of phenanthrene.
YANG Ren-jie, SUN Xue-shan, WANG Bin, DONG Gui-mei, YANG Yan-rong, ZHOU Chang-hong, ZHANG Wei-yu, LIU Hai-xue. 土壤湿度对多环芳烃荧光特性的影响[J]. 光谱学与光谱分析, 2017, 37(04): 1152-1156.
杨仁杰, 孙雪杉, 王 斌，董桂梅, 杨延荣，周长宏，张伟玉，刘海学. Effect of Soil Moisture Content on the Fluorescence Characteristics of Polycyclic Aromatic Hydrocarbons. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2017, 37(04): 1152-1156.
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