Determination of H2S in Rat Intestinal Perfusion Solution Based on Fluorescence Analysis
HOU Jun-feng1, LI Xin-xia2, SHEN Xue-ru1, Miliban Huojia1, GUAN Ming1*
1. Laboratory on Polmution Monitoring and Control, College of Chemistry and Chemical Engineering, Xinjiang Normal University, Urumqi 830054，China 2. College of Pharmacy of Xinjiang Medical University, Urumqi 830000, China
Abstract：Under alkaline conditions, Fluorescein mercury has strong fluorescence, however, when it met S2-, its fluorescence would quench, in view of the above, a fluorescence method for determination of H2S in biological samples was established. In the 0.1 mol·L-1 NaOH dilution, when the concentration of fluorescein Mercury and Na2S was 5.0×10-5 and 1.0×10-5 mol·L-1 respectively, the fluorescence intensity of system was determined at 522 nm. The results showed that, at the range of 4.0×10-7~2.0×10-6 mol·L-1, the concentration decreasing of H2S and fluorescence intensity had good linear relationship, r=0.998 0, the RSD of precision test was 4.59% (n=7), the detection limit was 3.5×10-8 mol·L-1, the content of H2S in the sample were 1.01×10-6 and 1.15×10-6 mol·L-1, and the recovery rate was 95.8%~101.0%, the method has the advantages of simple operation, high sensitivity, good selectivity, can accurately determine of H2S in intestinal perfused solution, and provides the basis for the determination of endogenous H2S.
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