Subcellular Distribution of Trace Elements in Wound Granulation Tissue of Severe Burn Patients by Inductively Coupled Plasma Mass Spectrometry
XU Xiang-rong1, XIE Hua-lin3*, FU Liang2,3, YANG Hua-juan1, HUANG Jian-hua2
1. The First People’s Hospital of Changde City, Changde 415003, China 2. School of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China 3. College of Chemistry and Chemical Engineering, Yangtze Normal University, Chongqing 408100, China
Abstract：A method for simultaneous and quantitative determination of Cr, Mn, Fe, Co, Cu, Zn, Se and Cd elements in the subcellular fractions of nuclei, mitochondria, lysosome, microsome and cytosol of wound granulation tissue of severe burn patients by octopole reaction system (ORS) inductively coupled plasma mass spectrometry (ICP-MS) was established. Using differential centrifugation, the sample is separated into different subcellular fractions. The subcellular fraction was digested by HNO3+H2O2 with microwave digestion followed by dilution with ultrapure water then the above 8 trace elements in the solution were analyzed directly by ICP-MS. In the presented method, using ORS eliminates the polyatomic interferences caused by the matrixes. Rh as internal standard element was used to compensate matrix effect and signal drift. The detection limits of the 8 elements are in the range of 0.72～33.05 ng·L-1, and the RSD is less than 8.4%. The results showed that the levels of some elements in subcellular fractions of wound granulation tissues were significantly different from those of normal skin tissues. ORS-ICP-MS is a useful tool for simultaneous determination of multi-elements in wound granulation tissue of severe burn patients, and could be widely used in other biological samples analysis.
Key words：Inductively coupled plasma mass spectrometry;Severe burn patients;Subcellular fraction;Trace elements
徐向荣1，谢华林3*，符 靓2,3，杨华娟1，黄建华2 . ICP-MS法研究重度烧伤患者创面肉芽组织中微量元素的亚细胞分布 [J]. 光谱学与光谱分析, 2014, 34(06): 1679-1682.
XU Xiang-rong1, XIE Hua-lin3*, FU Liang2,3, YANG Hua-juan1, HUANG Jian-hua2 . Subcellular Distribution of Trace Elements in Wound Granulation Tissue of Severe Burn Patients by Inductively Coupled Plasma Mass Spectrometry . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2014, 34(06): 1679-1682.
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