Determination of Arsenic Speciation in Scomberomorus Niphonius by Capillary Electrophoresis-Inductively Coupled Plasma Mass Spectrometry
CHEN Fa-rong1, 2, ZHENG Li2, WANG Zhi-guang2, SUN Jie1, HAN Li-hui1, WANG Xiao-ru2*
1. College of Chemistry and Chemical Engineering, Ocean University of China, Qingdao 266100, China 2. First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China
Abstract：A method for the detection of arsenocholine(AsC), arsenobetaine(AsB), As(Ⅲ), dimethylarsinic(DMA), monomethylarsonic(MMA) and As(Ⅴ) by capillary electrophoresis-inductively coupled plasma mass spectrometry(CE-ICP-MS) was established. The results showed that the six species of arsenic were separated within 20 min under the optimized conditions. Good linearities of 6 arsenic species were observed in the range from 2 to 50 μg·L-1 with the linear correlation greater than 0.996, the detection limits were 0.10~1.08 μg·L-1 and the RSDs (n=5) of the peak areas were smaller than 7%. The method was successfully adopted to the determination of the species in scomberomorus niphonius. The recoveries were between 93% and 98%, and we found the arsenobetaine(AsB) was the main species in the sample. The method was suitable for the analysis of other biological samples with the advantages of good stability, less sample consumption, short analysis time and convenience.
[1] Brisbin J A, B’Hymer C, Caruso J A. Talanta, 2002, 58: 133. [2] Le X C, Lu X F, Li X F. Arsenic speciation. Anal. Chem., 2004, 76: 27. [3] Zhang X, Cornelis R, Kimpe J D, et al. Clinical Chemistry, 1996, 42(8): 1231. [4] ZHANG Lei, ZHOU Rui, LI Xiao-wei, et al (张 磊, 周 蕊, 李筱薇, 等). Chin. J. Prev. Med.(中华预防医学杂志), 2008, 42(5): 298. [5] REN Feng-lian, MENG Jie, WU Yuan-xiong, et al(任凤莲, 孟 杰, 吴元雄, 等). Metallurgical Analysis(冶金分析), 2009, 29(2): 19. [6] WANG Geng, JING Miao, CAO Xuan, et al(王 庚, 荆 淼, 曹 煊, 等). Chinese J. Anal. Chem.(分析化学研究报告), 2008, 36(9): 1182. [7] YU Jing-jing, CAO Xuan, CUI Wei-gang, et al(余晶晶, 曹 煊, 崔维刚, 等). Food Science(食品科学), 2009, 30(16): 223. [8] Timerbaev A R. Talanta, 2000, 52: 573. [9] Olesik J W, Kinzer J A, Olesik S V. Anal. Chem., 1995, 67: 1. [10] CHEN Yu-hong, MI Jian-qiu, XU Lu-zheng, et al(陈玉红, 米健秋, 徐陆正, 等). Environmental Chemistry(环境化学), 2011, 30(7): 1374. [11] Yang G D, Xu J H, Zheng J P, et al. Talanta, 2009, 78: 471. [12] KANG Jian-zhen, DUAN Tai-cheng, LIU Jie, et al(康建珍, 段太成, 刘 杰, 等). Chinese J. Anal. Chem.(分析化学), 2004, 32: 262. [13] Taylor K A, Sharp B L, Lewis D J, et al. J. Anal. At. Spectrom., 1998, 13：1095. [14] Lu Q, Bird S M, Barnes R M. Anal. Chem., 1995, 67: 2949. [15] Borak J, Hosgood H D. Regulatory Toxicology and Pharmacology, 2007, 47: 204.
