Visualization of the Age-Related Changes in Expressions of DNA Methyltransferases in Mouse Oocytes Using Two-Photon Imaging System
TIAN Ning1, ZHANG Lu2, ZHENG Jing-hao1, LI Ying2, MA Wan-yun1*
1. State Key Laboratory of Low-Dimensional Quantum Physics, Department of Physics, Tsinghua University, Beijing 100084, China 2. Department of Histology and Embryology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, China
Abstract：Quantum dot (QD) is widely used as fluorescent labeling dye for its strong anti-fluorescence quenching, high quantum yield, wide absorption spectra, and narrow emission spectra. In the present paper, QD 585-labeled DNA methyltransferases (Dnmts) and Hoechst 33342-labeled chromosomes were imaged simultaneously using two-photon imaging system. The authors’ results showed that aging mouse oocytes may be not suitable for in-vitro maturation: both the localizations and expression levels of Dnmts in in-vitro matured oocytes of aging mice were changed, and these changes may be related to the abnormal DNA methylation modification.
田 宁1，张 露2，郑京镐1，李 英2，马万云1* . 双光子成像观测衰老对小鼠卵母细胞内DNA甲基转移酶表达的影响 [J]. 光谱学与光谱分析, 2012, 32(12): 3175-3178.
TIAN Ning1, ZHANG Lu2, ZHENG Jing-hao1, LI Ying2, MA Wan-yun1* . Visualization of the Age-Related Changes in Expressions of DNA Methyltransferases in Mouse Oocytes Using Two-Photon Imaging System. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2012, 32(12): 3175-3178.
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