壳聚糖包覆的CdTe量子点荧光猝灭法测定吉米沙星

doi:10.3964/j.issn.1000-0593(2012)06-1570-05

摘要
参考文献
相关文章 (15)

全文: PDF (2095 KB)
输出: BibTeX | EndNote (RIS)

摘要：以壳聚糖包覆的CdTe量子点为荧光探针，基于荧光猝灭法建立了吉米沙星定量测定方法。结果表明，体系的荧光强度与吉米沙星浓度在3.46×10^-9～3.46×10^-7 g·L^-1范围内呈良好的线性关系(r=0.999 2)，线性回归方程为F₀/F=1.063 7+0.016 7c(g·L^-1)。对2.77×10^-7 g·L^-1吉米沙星溶液进行7次平行测定的相对标准偏差为2.7%，基于荧光猝灭法相关理论证明了该相互作用过程为静态猝灭。本方法灵敏度高，检测线性范围宽，为吉米沙星定量测定提供了简便可靠的方法。

关键词：壳聚糖;CdTe量子点;荧光猝灭法;吉米沙星

Abstract：Chitosan-coated CdTe quantum dots can reduce QDs toxicity and enhance its stability in aqueous solution. Chitosan-coated CdTe QDs were used as fluorescence probes to determine Gemifloxacin on the basis of fluorescence quenching method. The results indicated that the relative fluorescence intensity was linearly proportional to the Gemifloxacin concentration in the range of 3.46×10^-9～3.46×10^-7 g·L^-1 with a linear fitting equation of F₀/F=1.0637+0.016 7c(g·L^-1) and the RSD was 2.7%. On the basis of fluorescence quenching method theory, it was concluded that the interaction between QDs and Gemifloxacin was a kind of static quenching through hydrogen bonding and Van der Waals force, and the binding sites value was 0.8. This method with high sensitivity and broad linear range provided a new approach to determining Gemifloxacin.

Key words：Chitosan;CdTe QDs;Fluorescence quenching method;Gemifloxacin

收稿日期: 2011-11-15 修订日期: 2012-02-28

中图分类号:

O657.3

通讯作者: 钟文英 E-mail: wyzhong@cpu.edu.cn

引用本文:

钟文英^1，2，王妍¹，黄斌¹，舒畅¹ . 壳聚糖包覆的CdTe量子点荧光猝灭法测定吉米沙星 [J]. 光谱学与光谱分析, 2012, 32(06): 1570-1574.
ZHONG Wen-ying^{1, 2}, WANG Yan¹, HUANG Bin¹, SHU Chang¹. The Quantification of Gemifloxacin by Fluorescence Quenching Method Using Chitosan-Coated CdTe Quantum Dots . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2012, 32(06): 1570-1574.

链接本文:

https://www.gpxygpfx.com/CN/10.3964/j.issn.1000-0593(2012)06-1570-05 或 https://www.gpxygpfx.com/CN/Y2012/V32/I06/1570