Abstract：In the present study, Cu(Ⅱ)-imprinted polymer was prepared and used as an effective method for separation and enrichment of Cu(Ⅱ) ion in samples. An on-line preconcentration and extraction flow-injection system using solid phase extraction (SPE) column packed with prepared ion imprinted polymer (IIP) was set up. The column was eluted with a mixture of ethanol and H2SO4 after preconcentration. A sensitive and simple chemiluminescence(CL) method using flow-injection has been developed for the determination of copper. The method is based on the fact that traces of Cu(Ⅱ) exert a catalytic action on the oxidation between morin by KMnO4 solution. After optimizing the different experimental parameters, a calibration graph was obtained over the concentration range of 0 to 10 μg·mL-1 for Cu(Ⅱ) and r=0.996 8. The detection limit is 0.001 2 μg·mL-1. The recoveries are between 95% and 105% and the standard relative deviations( RSD) are less than 3% at 4.0 μg·mL-1 (n=9) level was obtained. The new chemiluminescence determination for copper has been established. The method can be satisfactorily applied to the determination of copper in sample.
[1] Rao T P, Daniel S, Gladis J M. Trends Anal. Chem., 2004, 23: 28. [2] Joseph M G, Tslasila P R. Analytical Letters, 2003, 36(10): 2107. [3] Kala R, Talasila P R. Separation Science and Technology, 2006, 41: 233. [4] ZHUANG Hui-sheng, CHEN Guo-nan, WANG Qiong-e, et al(庄惠生，陈国南，王琼娥，等). Chinese J. Anal. Chem.(分析化学), 2000, 28(5): 573. [5] WANG Ting, XIONG Yu-bao, GAO Xiao-hong, et al(王 婷，熊玉宝，高小红，等). Metallurgical Analysis(冶金分析), 2007, 27(12): 50. [6] Bingchun X, Tao W, Erbao L. J. Anal. Chem., 2008, 63(11): 1078. [7] FAN Hong-tao, SUN Ting, DONG Jia, et al(范洪涛，孙 挺，董 佳, 等). Chemistry(化学通报), 2009, 72(1): 10. [8] ZHU Jian-hua，LI Xin，QIANG Liang-sheng(朱建华, 李 欣, 强亮生). Chem. J. Chinese Universities(高等学校化学学报)，2006, 27(10): 1853. [9] LAI Xiao-qi, YANG Yuan-qi, XUE Jun(赖晓琦，杨远奇，薛 珺). Acta Chem.Sinica(化学学报), 2009, 67(8): 863. [10] Mohammad S, Hamideh Y. Journal of Hazardous Materials, 2009,167: 1152. [11] Cem E, Muge A, Nilay B, et al. Materials Science and Engineering C, 2009, 29: 2464. [12] Dhruv K S, Shraddha M. Anal Chem. Acta, 2009, 644: 42. [13] Neil W B, Benjamin J H, Philip J, Trevor A S. Anal. Chem. Acta, 2002, 451: 181.
