Abstract:A new, rapid and sensitive method for the determination of bovine serum albumin (BSA) by flow-injection coupled with chemiLuminescence detection was establish, which based on BSA enhancing effect on weak chemiLuminescence system of Luminol-H2O2 in alkaline solution. Under the optimized conditions, linearity for BSA was found in the range of 2.5×10-9~1.0×10-6 mol·L-1, with the detection limit of 5.8×10-10 mol·L-1. And the sampling detecting rate was about 112 samples·h-1. The relative standard deviation(RSD)was found to be 0.8% for 11 replicate determinations of 1.0×10-7 mol·L-1 BSA. The proposed method has been satisfactorily applied to the determination of BSA in the bovine serum samples. The possible mechanism of Luminol-H2O2-BSA was studied by chemiLuminescence spectrum and UV-visible spectrum.
杜俊俊1,王 辉1,任书伟2,周 敏1,刘迎迎1,刘彦明1* . 流动注射化学发光法灵敏检测牛血清白蛋白 [J]. 光谱学与光谱分析, 2013, 33(06): 1693-1696.
DU Jun-jun1, WANG Hui1, REN Shu-wei2, ZHOU Min1, LIU Ying-ying1, LIU Yan-ming1* . Sensitive Determination of Bovine Serum Albumin by Flow-Injection Chemiluminescence . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2013, 33(06): 1693-1696.
[1] Guo Z X,Shen H X. Analyst,1999,7(124):1093. [2] Wang L Y,Zhou Y Y,Wang L,et al. Anal. Chim. Acta,2002,466(1): 87. [3] WANG Jun-tao,SONG Shuang-ju,WU Qiu-hua,et al(王珺瑫,宋双居,吴秋华,等). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2011,31(9):2517. [4] SUN Wei,JIAO Kui,LIU Xiao-yun(孙 伟,焦 奎,刘晓云). Chinese Journal of Analytical Chemisty(分析化学),2002,30(3): 312. [5] ZHANG Meng-juan,GUO Yan-li,YAN Hong-tao,et al(张梦娟,郭艳丽,阎洪涛,等). Journal of Analytical Science(分析科学学报),2011,27(1):1. [6] Tang D P,Niessner R,Knopp D. Biosens. Bioelectron.,2009,24(7): 2125. [7] Liu Y M,Liu Z L,Shi Y M. Luminescence,2011,26(1): 59. [8] Shitanda I,Takamatsu S,Watanabe K,et al. Electrochim. Acta,2009,54(21): 4933. [9] Liu Y M,Mei L,Liu L J,et al. Anal. Chem.,2011,83(3): 1137. [10] Li R B,Kameda T,Toriba A,et al. Anal. Chem.,2012,84(7): 3215. [11] Chen L L,Zhang Z J,Zhang X M,et al. Food Chem.,2012,135(1): 208. [12] Revello M G,Gorini G,Gerna G. Clin. Vaccine. Immunol.,2004,11(4): 801. [13] Ma L,Kang W J,Xu X D,et al. J. Anal. Chem.,2012,67:219. [14] Qi H L,Li S G,Lin L,et al. Spectrochim. Acta A,2011,82(1): 498. [15] Tan X J,Song Z H,Chen D H,et al. Spectrochim. Acta A,2011,79(1): 232.