Abstract：Organic autotrophic particles were detected by flow cytometry(FCM) in an oligotrophic lake. Utilizing scattering signals and fluorescence of their natural pigments, different populations could be rapidly discriminated and enumerated. We found that they belonged to three populations. R1 were the smallest population, while R3 were the largest one. R1 contained phycoerythrin but lacked chlorophyll. On the contrary, R3 contained a large amount of chlorophyll but lacked phycoerythrin. R2 contained not only chlorophyll but also a great deal of phycoerythrin. Organic autotrophic particles account for the most proportion of the total particles that were smaller than 50 μm. They represented 70.24% of the total particles. In this study, we found that flow cytometry is particularly suited to the analysis of organic autotrophic particles that are difficult to study with traditional methods.
[1] Moreira-turcq P, Martin J M, Fleury A. Mar. Chem., 1993, 43: 115. [2] Chisholm S W, Olson R J, Zetter E R et al. Nature, 1988, 334: 340. [3] Vaulot D, Marie D, Olson R J et al. Science, 1995, 268: 1480. [4] Blanchot J, Andre J M, Navarette C et al. Deep-Sea Research Ⅰ, 2001, 48: 297. [5] GUO Pei-yong, SHEN Huan-ting, ZHANG Li-hua(郭沛涌，沈焕庭，张利华). Acta Biophysica Sinica(生物物理学报), 2002, 18(3): 359. [6] SONG Ping-gen, LI Xiu-wen(宋平根，李修文). Principles and Application of Flow Cytometry(流式细胞术的原理和应用). Beijing(北京): Beijing Normal University Press(北京师范大学出版社), 1992. 7. [7] Marie D, Partensky F, Vaulot D et al. In Robinson J P. Current Protocols in Cytometry. New York: John Wiley & Sons Inc, 1999.
