Abstract：In pH 7.40 Tris-HCl buffer solution, PMA is light yellow and has no absorption peak in the visible region. HSA has no color and no absorption peak in the visible region either. There is a fluorescence peak at 350 nm for HSA in pH 7.40 Tris-HCl buffer solution. HSA and PMA can combine into HSA-PMA association nanoparticles with a diameter about 80 nm by means of static gravitation. HSA has both hypochromic and color enhancement effects on PMA through our research. The fluorescence of Trp, Tyr and HSA can be quenched by PMA, but their quenching mechanisms are different. The fluorescence quenching of Trp and Tyr by PMA is due to molecule absorption in the range of emission wavelength，namely because of energy transfer often reported. The investigation results demonstrated that the formation of HSA-PMA associated nanoparticle and interface is the ultimate reason for fluorescence quenching, resonance scattering, and enhanced and hypochromic color.
Key words：HSA-PMA associated nanoparticle;Fluorescence quenching;Hypochromic and enhanced color effect;Enhanced scattering effect
[1] ZENG Yi(曾 溢主编). The Hereditary Disease for Protein and Nucleic Acid(蛋白质和核酸遗传病). Shanghai: Shanghai Press(上海：上海出版社)，1981. 114. [2] Peters T，Sjobholm I. FEBS, 11th Meeting Copenhagen Ｖolume 50 Colloquim B9, 1977. 1, 50. [3] Ochiai E，Williams D R. Laboratory Introduction to Bio-inorganic Chemistry. Lond，Macmillan, 1979: 34. [4] TANG Ren-huan(唐任寰主编). The Weight of Equilibrating Life-Microelements and Health(平衡生命的砝码-微量元素与健康). Changsha: Hunan Education Press(长沙：湖南教育出版社)，1988. 1. [5] Todd L. Where There's Life, There's Phosphorus. In: Makoto K, Tairo O, eds. Science and Scientists. Tokyo: Japan Sci. Soc. Press, 1981. 275. [6] ZHANG Bao-lin, WANG Wen-qing, BAI Feng-lian(张保林，王文清，白风莲). Chem. J. Chinese Universities(高等学校化学学报), 1994, 15(3): 373. [7] WANG Li-sheng, JIANG Zhi-liang et al(王力生，蒋治良等). J. Guangxi Normal University(广西师大学报)，2002, 20(2)：114. [8] JIANG Zhi-liang, LIU Shao-pu，JIANG Hong-liu(蒋治良，刘绍璞，江洪流). Chin. J. Appl. Chem.(应用化学)，2002, 19: 1133. [9] LIANG Ai-hui，JIANG Zhi-liang, WANG Li-sheng et al(梁爱惠，蒋治良，王力生等). Chin. J. Appl. Chem.(应用化学)，2001，18：867. [10] Fiabane A M，Willians D R. The Principles of Bio-inorganic Chemistry(无机生物化学原理). Trans. by Huang Z X, Xu X. Shanghai: Fudan University Press(上海：复旦大学出版社)，1984. 12. [11] JI Liang-nian, HUANG Jin-jiang et al(计亮年, 黄锦江等). Introduction of Bio-Inorganic Chemistry (2nd edition)(生物无机化学导论(第二版)). Guangzhou: Zhongshan University Press(广州：中山大学出版社)，2001. 225. [12] GUO Xiao-jun(郭晓君主编). The Experimental Technique of Fluorescence and Its Application in Biochemistry(荧光实验技术及其在分子生物学中的应用). Beijing: Science Press(北京：科学出版社)，1979. 113. [13] Froehlich P M et al. Anal. Chem.，1977, 49：1606.
