Abstract：Experiments indicated that terbium(Ⅲ) ion could form complex with norepinephrine, then emitted its characteristic fluorescence. In the present paper, the fluorescence reaction of terbium(Ⅲ) ion with norepinephrine was studied in detail. Thus a new method for the determination of norepinephrine was propsed. The fluorescence intensity was measured in a 1 cm quartz cell with the excitation and emission wavelengths of 300 and 545 nm, respectively. The result showed that the fluorescence intensity of the system presented a linear relationship with the concentration of norepinephrine in the range of 0.01-50 μg·mL-1, and the detection limit(S/N=3) was 1.0 ng·mL-1. The method was used to determine pharmaceutical sample which contained norepinephrine, and the result was satisfactory.
[1] TANG Qin-mei(唐琴梅). Chinese Journal of Chromatography(色谱)，1992，10(2)：82. [2] Kagedal B, Goldstezn D S. J. Chromatogra., 1988, 429: 177. [3] Merrills R J. Nature, 1962, 193: 988. [4] Seki T. J. Chromatogra., 1978, 155: 415. [5] Curtius H Ch, Wolfensberger M, Steinmann B. Journal of Chromatography, 1974, 99: 529. [6] Oosterhuis B, Brunt K, Westerink B H C. Anal. Chem., 1980, 52: 203. [7] TONG Chang-lun，ZHU Yan，GUO Dan，XIANG Guang-hong，YE Ming-li(童裳伦，朱 岩，郭 丹，项光宏，叶明立). Chinese Journal of Analytical Chemistry(分析化学)，2001，29(10)：1237. [8] TONG Chang-lun，ZHU Yan，YANG Jing-he(童裳伦，朱 岩，杨景和). Chinese Journal of Analytical Chemistry(分析化学)，2000，28(3)：293. [9] YANG Jing-he，TONG Chang-lun，JIE Nian-qin，GONG Hong-yu, GAO Zu-quan(杨景和，童裳伦，揭念琴，龚红宇，高祖泉). Chinese Biochemical Journal(生物化学杂志)，1996，12(2)：143.
