The Study on the Fluorescence Spectrometry for a Novel Photosensitizer of Chlorine e6-C15-Monomethyl Ester and Its Application in Biosamples Analysis
YANG Dan1, ZHAO Xin2, 3, SUN Fan-lu2, 3, QI Tian2, 3, LIU Cheng-chu1*, FAN Guo-rong2, 3*
1. College of Food Science & Technology, Shanghai Ocean University, Shanghai 201306, China 2. Department of Pharmaceutical Analysis, School of Pharmacy, Second Military Medical University, Shanghai 200433, China 3. Shanghai Key Laboratory for Pharmaceutical (Chinese Materia Medica) Metabolite Research, Shanghai 200433, China
Abstract：This paper studied the fluorescence spectral characteristic of chlorin e6-C15-monomethyl ester in different solvents to develop a fluorescence spectrometry for determining the concentration of chlorin e6-C15-monomethyl ester in plasma. By comparing the fluorescence spectral characteristics of chlorin e6-C15-monomethyl ester in six different solvents including methanol, ethanol, acetone, acetonitrile, phosphate buffered saline and water, the influence of different solvents on the fluorescence spectral characteristic of chlorin e6-C15-monomethyl ester has been examined. The results indicated that methanol and acetonitrile are the ideal solvent system, and the effect of phosphate buffered saline is better than water. The effect of organic solvent content and solution pH value on emission wavelength and fluorescence intensity was further evaluated. It was found that the fluorescence intensity of chlorin e6-C15-monomethyl ester was strong and stable in pH 7.2 phosphate buffered saline -acetonitrile (3∶7) solution and can be detected at the excitation wavelength of 498.00 nm and the emission wavelength of 664.05 nm. Based on this, we developed the fluorescence spectrometry for determining chlorin e6-C15-monomethyl ester. It is specific and sensitive with good linearity over the range of 0.5～50 μg·mL-1. The intra-batch and inter-batch precisions (RSD) were less than 10%, the extraction recoveries were all over 90%. The established method in this paper was simple, fast, effective and could be successfully applied to study the pharmacokinetics of chlorine e6-C15-monomethyl ester in SD rats.
杨 旦1，赵 鑫2, 3，孙范露2, 3，齐 天2, 3，刘承初1*，范国荣2, 3* . 新型光敏剂二氢卟吩e6-C15单甲酯荧光光谱特性及其体内分析应用 [J]. 光谱学与光谱分析, 2014, 34(04): 1007-1011.
YANG Dan1, ZHAO Xin2, 3, SUN Fan-lu2, 3, QI Tian2, 3, LIU Cheng-chu1*, FAN Guo-rong2, 3* . The Study on the Fluorescence Spectrometry for a Novel Photosensitizer of Chlorine e6-C15-Monomethyl Ester and Its Application in Biosamples Analysis. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2014, 34(04): 1007-1011.
[1] Ryuji Asano，Amon Nagami，Yuki Fukumoto，et al. Bioorganic & Medicinal Chemistry，2013，21：2298. [2] Shigenobu Yano，Shiho Hirohara，Makoto Obata，et al. Journal of Photochemistry and Photobiology C: Photochemistry Reviews，2011，12：46. [3] Ron R Allison，Gordon H Downie，Rosa Cuenca，et al. Photodiagnosis and Photodynamic Therapy，2004，1：27. [4] YU Ke-gui，ZHOU Cheng-he，LI Dong-hong(于克贵，周成合，李东红). Chemical Research and Application(化学研究与应用)，2007，19(12)：1296. [5] FANG Ying，XU De-yu(方 瑛，许德余). Chinese Journal of Laser Medicine & Surgery (中国激光医学杂志)，1999，8(4)：235. [6] CHEN Guo-zhen(陈国珍) . Fluorescence Spectrometry(荧光分析法). Beijing: Science Press(北京：科学出版社)，2006. [7] Wang Rui，Hao Haiping，Wang Guangji，et al. Journal of Photochemistry and Photobiology B: Biology，2008，90：179. [8] WANG Lu，CHEN Yan，ZHU Si-jia，et al(王 璐，陈 雁，朱思佳，等). Journal of Shanxi Normal University: Natural Science Edition(陕西师范大学学报·自然科学版)，2012，40(2)：71. [9] WANG Qi，ZHUANG Yan，YE Xiao-hui(王 琪，庄 严，叶小会). Chinese Journal of Spectroscopy Laboratory (光谱实验室)，2010，27(6)：2197. [10] Pharmacopiea Committee of the Ministry of Health of the People’s Republic of China(中华人民共和国国家药典委员会). Pharmacopiea the People’ Republic of China(中华人民共和国国家药典). Beijing：The Medicine Science and Technology Press of China(北京：中国医药科技出版社)，2010. [11] U.S. Food and Drug Administration. FDA Guidance for Industry: Bioanalytical Method Validation. United States of America, 2001. [12] FANG Ying，XU De-yu(方 瑛，许德余). Organic Chemistry(有机化学)，1995，15(5)：493. [13] WANG Ke-dong，MIAO Tong-jun，ZHAO Xiao-li(王克栋，苗同军，赵晓利). Journal of Xinxiang University：Natural Science Edition(新乡学院学报·自然科学版)，2011，28(2)：127. [14] SUN Yan-chun(孙延春). Chinese Journal of Spectroscopy Laboratory(光谱实验室)，2009，26(6)：1590.
