Study on the Interaction of Anticancer Drugs and DNA Using Fluorescence Spectroscopy
SHEN Jing-shan1, SUN Dan-dan2, FU Lian-chun4, Lü Wen-bo1, Lü Guo-wei1, YE Xue-min1, MENG Guang-zheng3, SONG Zeng-fu1*
1. School of Physics, Peking University, Beijing 100871, China 2. College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China 3. Central Iron and Steel Research Institute, Beijing 100081, China 4. CTA-Tex Chemicals, Beijing 100025, China
Abstract：Fluorescence spectroscopy has achieved great successes in the study on the interaction of drugs and DNA in both quantitative analysis and qualitative analysis. While the fluorescence probe technique can be used to measure the intensity of drugs-DNA interaction, because the injected drugs can change the fluorescence intensity of the probe. In this paper, the authors use berberine as a probe to measure the interaction intensity between some anticancer drugs and DNA, calculate the constant D and determine the effects of those drugs. In addition, the authors point out that this method can not be efficient for certain drug solution.
沈景山1，孙丹丹2, 付连春4, 吕文波1，吕国伟1，叶学敏1，孟广政3，宋增福1* . 荧光光谱法研究抗癌药物与DNA的相互作用[J]. 光谱学与光谱分析, 2005, 25(02): 232-234.
SHEN Jing-shan1, SUN Dan-dan2, FU Lian-chun4, Lü Wen-bo1, Lü Guo-wei1, YE Xue-min1, MENG Guang-zheng3, SONG Zeng-fu1* . Study on the Interaction of Anticancer Drugs and DNA Using Fluorescence Spectroscopy . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2005, 25(02): 232-234.
