The Research on the Quenching of Fluorescence Spectra of TOPⅠ by Iodine Anion
XIA Bing-le1, PENG Dun-geng1, LI Min-li1, XIAO Hong2, WANG Kai-bao3
1. Department of Chemistry, USTC，Hefei 230026, China 2. Zhumadian Cigarette Factory，Zhumadian 463000, China 3. Wuhu Cigarette Factory，Wuhu 241000, China
Abstract：Comparing the fluorescence spectra of tobacco peroxidaseⅠ(TOPⅠ) solution and the solutions titrated by iodine anions，the number of binding locus and the binding constant of iodine anion to TOPⅠ were calculated by using the modified Stern-Volmer equation. The mechanism of the quenching of fluorescence spectra and the distribution of tryptophane residues in the TOPⅠ molecule were discussed.
Key words：Tobacoo peroxidase Ⅰ;Quenching mechanism;Number of binding locus;Binding constant
夏炳乐1，彭敦耕1，李敏莉1，肖 洪2，王开保3 . 碘离子对烟草过氧化物酶Ⅰ(TOPⅠ)的荧光猝灭的研究[J]. 光谱学与光谱分析, 2005, 25(02): 239-241.
XIA Bing-le1, PENG Dun-geng1, LI Min-li1, XIAO Hong2, WANG Kai-bao3 . The Research on the Quenching of Fluorescence Spectra of TOPⅠ by Iodine Anion. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2005, 25(02): 239-241.
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