Spectrophotometric Determination of Trace Amount of Copper with Trimethoxyphenylfluorone in Microemulsion after Preconcentration and Separation by Sulphydryl Dextran Gel
WEI Qin1, DU Bin1, WU Dan1, OU Qing-yu2
1. School of Chemistry and Chemical Engineering, Ji'nan University, Ji’nan 250022, China 2. Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China
Abstract：In this paper, the color reaction of copper with trimethoxyphenylfluorone (TM-PF) in microemulsion has been studied. Copper (Ⅱ) reacts with TM-PF to form a stable 1∶2 complex at pH 10.40. Its apparent molar absorptivity is 1.46×105 L·mol-1·cm-1 at 552 nm. Beer’s law is obeyed in the range of 0-0.6 μg·mL-1. Sulphydryl dextran gel (SDG), in which sulphydryl group has a big share, is a new type of metallic ion adsorbent. It presents the advantage of higher adsorption capacity, better reuse ability and higher mechanical stability. So the coexistent metal ions can be separated effectively and copper can be enriched by SDG, greatly improving the selectivity and sensibility of the system. The method was used to determine trace amount of copper in blood, urine, iron ore and water samples with satisfactory results, the relative standard deviation is less than 4.8%, and the recovery is 94.2%-106%.
Key words：Trimethoxyphenylflurone;Copper(Ⅱ);Microemulsion;Biosamples;ore and water samples;Sulphydryl dextrane gel;Preconcentration and separation;Spectrophotometry
魏 琴1，杜 斌1，吴 丹1，欧庆瑜2 . 巯基葡聚糖凝胶分离富集微乳液增敏三甲氧基苯基荧光酮光度法测定痕量铜[J]. 光谱学与光谱分析, 2005, 25(05): 772-775.
WEI Qin1, DU Bin1, WU Dan1, OU Qing-yu2 . Spectrophotometric Determination of Trace Amount of Copper with Trimethoxyphenylfluorone in Microemulsion after Preconcentration and Separation by Sulphydryl Dextran Gel. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2005, 25(05): 772-775.
