Studies on TCs-Fe(Ⅲ) System Separation/Enrichment of Trace Tetracyclines Antibiotic by Combination Ionic Liquid [Hmim]PF6 Solvent Sublation with Fluorescence Spectroscopy Determination
WANG Liang1,2, YAN Yong-sheng2*, LI Hua-ming2, SUN Han-wen3
1. College of Chemistry, Jilin Normal University，Siping 136000, China 2. College of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, China 3. College of Chemistry & Environmental Science, Hebei University, Baoding 071000, China
Abstract：It is the first time ionic liquid was applied to gas solvent sublation, which established a new method for separation/enrichment of tetracyclines(TCs) antibiotic based on ionic liquid gas solvent sublation. The optimizing experimental conditions were as follows: the mixture of ionic liquid 1-hexyl-3-methylimidazo lium hexafluorophosphats and ethy1 acetate ([Hmim]PF6-EA，φ=1/0.9) can be used as flotation solvent, Fe(Ⅲ) ion was as trapping agent, pH of test solution was 7.6, the gas flow rate was 40 mL·min-1, and the flotation time was 50 min. The complexes of TCs- Fe(Ⅲ) were pre-concentrated in the [Hmim]PF6-EA layer and this was used to determine analytes by fluorescence method directly. The linear regression equation was F=246.5c+4.32 (c: μg·10 mL-1), and correlation coefficient was 0.999 1. Recoveries between 94.2% and 100.4% were achieved from surface water and sediment samples in ponder by using this method and the relative standard deviation of 5 μg·mL-1 TCs for 5 parallel determinations was less than 3.2%. FTIR spectroscopy demonstrated that no chemical (bonding) interactions occurred between the ionic liquid and the complexes functional groups, and the ionic liquid was only solvent. The experimental result indicated that utilizing ionic liquid to float TCs in environmental sample is feasible.
王 良1，2，闫永胜2*，李华明2，孙汉文3 . TCs-Fe(Ⅲ)体系离子液体气浮浮选荧光光谱法分离/富集四环素类抗生素残留的研究[J]. 光谱学与光谱分析, 2010, 30(02): 391-394.
WANG Liang1,2, YAN Yong-sheng2*, LI Hua-ming2, SUN Han-wen3 . Studies on TCs-Fe(Ⅲ) System Separation/Enrichment of Trace Tetracyclines Antibiotic by Combination Ionic Liquid [Hmim]PF6 Solvent Sublation with Fluorescence Spectroscopy Determination . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2010, 30(02): 391-394.
[1] Patrícia Penido Maia, Ernani Clarete da Silva, Susanne Rath. Food Control., 2009, 20(1): 11. [2] Masakazu H, Harumi T, Kazuo T. Anal. Chim. Acta, 2003, 492(1-2): 187. [3] Carmen L, Rosa C, Ramon C. J Chromatogr. A, 2001, 910(2): 285. [4] Schneider M J, Braden S E, Reyes-Herrera I. J. Chromatogr. B, 2007, 846(1-2): 8. [5] Spisso B F, Oliveira Jesus A L, Araújo MA Jr. Anal. Chim. Acta, 2007, 581(1): 108. [6] SHAN Hai-xia, LI Zai-jun(单海霞, 李在均). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2008, 28(1): 214. [7] Visser A E, Swatloski R P, Reichert W M. Environ. Sci. Technol., 2002, 36(11): 2523. [8] Liu Jingfu, Jiang Guibin. Anal. Chem., 2003, 75(21): 5870. [9] Soto A, Are A. Separation and Purification Tech., 2005, 44(3): 242. [10] Huddleston J G, Willauer H D, Rogers R D. Chem. Commun., 1998, (16): 1765. [11] Khachatryan Kristine S, Smirnova Svetlana V. Anal. & Bioanal. Chem., 2005, 381(2): 464. [12] Cheng Qun, Dong Hui-ru, Microchimica Acta, 2005, 150: 59. [13] Kim Young-Sang, Shin Je-Hyuk, Choi Yoon-seok. Microchemical Journal, 2001, 68: 99. [14] Gu Z, Brennecke J F. J. Chem. Eng. Data, 2002, 47(2): 339. [15] Seddon K R, Stark A, Tores M J. Pure and Appl. Chem., 2000, 72: 2275. [16] Wang Ji-jiang, Zhu An-lian, Zhao Y. Journal of Solution Chemistry, 2005, 34: 585. [17] WANG Liang, YAN Yong-sheng, LI Hua-ming, et al(王 良, 闫永胜, 李华明, 等). Chinese Journal of Analytical Chemistry(分析化学), 2009, 37: 72. [18] WEN Mei-qiong, LI Lu(文美琼, 李 露). Chinese Journal of Spectroscopy Laboratory(光谱实验室)，2008, 25: 226.
