Abstract：The localization of activity of glucose oxidase (GOD) on the pussian blue modified electrode by SiO2 sol-gel was studied by X-ray microanalysis.Glucose and Ce(NO3)3 served as substrate and capture respectively.Substrate was catalysed by immobilized GOD to produce H2O2，and the H2O2 was captured by Ce(NO3)3 to form precipitate.The precipitation deposited the active site of immobilized GOD on the enzyme electrode.The X-ray microanalysis showed that the distribution of active enzyme on the surface of the enzyme electrode was uniform, and the GOD immobilized maintained high activity.The X-ray analysis exhibited that the properties of the enzyme electrode depended on the distribution of enzyme activity on its surface from the view of microstructure, and the developed GOD electrode was of high sensitivity and excellent stability.The results agreed with the results of the electrochemical study.
李 彤，姚子华*，仇满德，安 伟，史大刚 . 酶电极上葡萄糖氧化酶的活性的X射线微区分析[J]. 光谱学与光谱分析, 2005, 25(07): 1151-1154.
LI Tong, YAO Zi-hua*, QIU Man-de, AN Wei, SHI Da-gang. X-Ray Microanalysis of the Activity of Immobilized Glucose Oxidase on Enzyme Electrode . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2005, 25(07): 1151-1154.
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