不同苯原酚化合物用于GOD-Trinder法测定血糖的比较研究

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摘要：对苯酚、2,4-二氯苯酚、2,6-二氯苯酚及间-苯二酚等四种苯原酚化合物作为过氧化物酶(POD)催化4-氨基安替比林(4-AA) -过氧化氢-酚显色体系测定血糖的葡萄糖氧化酶法(GOD-Trinder)进行比较研究。结果表明，苯酚、2,4-二氯苯酚及间-苯二酚三种苯原酚分别构成GOD-POD-4-AA-H₂O₂-酚显色体系测定血糖时，其检测结果均无显著性差异(P＞0.05)，但2,4-二氯苯酚显色体系的显色灵敏度、方法的精密度和准确度均优于苯酚显色体系。间-苯二酚显色体系灵敏度不及苯酚，但检测速度快，线性范围宽，可用于较高浓度葡萄糖溶液的在线快速分析。

关键词：葡萄糖;血清;葡萄糖氧化酶;过氧化物酶;生物化学检验

Abstract：Comparative studies were carried out on GOD-trinder method for measuring glucose in serum with four different kinds of benzene-original phenols-phenyl hydroxide, 2,4-dichlorophenol, 2,6-dichlorophenol and resorcinol. It is shown that when the three coloring systems-phenyl hydroxide, 2,4-dichlorophenol or resorcinol coupling with glucose oxidase-peroxidase-4-a minoantipyrine-hydrogen peroxide (GOD-POD-4-AA-H₂O₂) respectively were used for clinical assay of glucose in serum, the determination results showed no significant differences (P＞0.05). Compared with phenyl hydroxide, the coloring system of 2,4-dichlorophenol is better in the aspects of sensitivity, precision and accuracy; the resorcinol system has lower sensitivity with the advantage of faster detecting velocity, and larger linear range, and can be used for on-line rapid analysis of samples with more concentrated glucose in industry.

Key words：Glucose;Serum;Glucose oxidase;Peroxidase;Biochemical assay

收稿日期: 2005-01-06 修订日期: 2005-04-10

中图分类号:

O613

通讯作者: 邓健

引用本文:

徐生¹,邓健^1*,许金生²,袁亚莉¹,张伟¹ . 不同苯原酚化合物用于GOD-Trinder法测定血糖的比较研究[J]. 光谱学与光谱分析, 2006, 26(03): 526-528.
XU Sheng¹,DENG Jian^1*,XU Jin-sheng²,YUAN Ya-li¹,ZHANG Wei¹ . Comparative Study on GOD-Trinder Method for Measuring Glucose in Serum with Different Benzene-Original Phenols. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2006, 26(03): 526-528.

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https://www.gpxygpfx.com/CN/Y2006/V26/I03/526