Abstract：The change in the UV-absorption spectrum and fluorescence spectrum of acridine orange(AO) due to the addition of surfactant dodecyi benzene sulfonic acid sodiumsait (SDBS) and bovine serum albumin(BSA) was studied. Meanwhile, the effects of the in situ formed AOAO dimer in SDBS as a fluorescence probe and BSA were discussed. A new method for the determination of BSA using fluorescence is presented. The results indicate that the method is sensitive and rapid. The linear range of determination is 0-4.17×10-7 mol·L-1. The relative standard deviation is 1.9%, and the detection limit is 8.73×10-10 mol·L-1.
石燕，郑为完，吴晓星，周德红. 吖啶橙与表面活性剂的作用及其在测定蛋白质中的应用[J]. 光谱学与光谱分析, 2006, 26(09): 1653-1655.
SHI Yan, ZHENG Wei-wan, WU Xiao-xing, ZHOU De-hong . The Interaction of Acridine Orange with Anionic Surfactant and Its Application to the Determination of Protein. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2006, 26(09): 1653-1655.
[1] XIAO Hou-rong，SHENG Liang-quan, SHI Chun-hua, et al(肖厚荣，盛良全，施春华, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2004，24(1)：78. [2] SHI Yan, YAN Yuan, HUANG Jian-feng(石 燕, 鄢 远，黄坚锋). Journal of Nanchang University(Natural Science)(南昌大学学报·理科版), 2000，24(3)：282. [3] GUO Xiang-qun, LI Fang, ZHAO Yi-bing, et al(郭祥群，李 芳，赵一兵, 等). Chem. J. Chinese Univ.(高等学校化学学报)，1996，17(9)：1361. [4] HE Xi-wen，FENG Xi-zeng, ZHANG Gui-zhu, et al(何锡文, 冯喜增，张贵珠, 等). Chinese J. of Anal. Chem.(分析化学)，1994，22(6)：565.
