Immune Resonance Scattering Spectral Method for the Determination of Trace IgG
DENG Jun-yao1，3, SUN Shuang-jiao1, JIANG Zhi-liang1，2*, LIANG Ai-hui2
1. Department of Resource and Environmental Science, Guangxi Normal University, Guilin 541004, China 2. Department of Material and Chemical Engineering, Guilin University of Technology, Guilin 541004, China 3. Children-Women Health Hospital of Guilin City, Guilin 541001, China
Abstract：In pH 7.0 Tris-HCl buffer solution, goat-anti-rabbit IgG is combined with rabbit IgG specifically, and aggregates to form immune complex particles that exhibit three resonance scattering peaks at 330, 400 and 520 nm respectively, and a synchronous scattering peak at 470 nm, in the presence of PEG-20000. The scattering intensity at 470 nm is linear to the rabbit IgG concentration in the range of 1.33 to 133.3 μg·mL-1. The detection limit is 0.99 μg·mL-1. The method was applied to the quantitative analysis of rabbit IgG, with satisfactory results.
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