Resonance Scattering Spectra of Apolipoprotein Immunocomplex Particles and Its Analytical Application
SUN Shuang-jiao1, 3,LIANG Ai-hui2,JIANG Zhi-liang1*,CHEN Bin1
1. School of Environment and Resource, Guangxi Normal University, Guilin 541004, China 2. Department of Material and Chemical Engineering, Guilin University of Technology, Guilin 541004, China 3. Shaoyang Medical College, Shaoyang 422000, China
Abstract：In pH 6.8 Na2HPO4-NaH2PO4 buffer solution and in presence of polyethylene glycol(PEG), apolipoprotein AI (ApoAI) and apolipoprotein B (ApoB) would combine with their corresponding antibody and produced immune complex particles in size of about 180 nm and 140 nm respectively, which exhibit stronger resonance scattering (RS) effect at 340 nm and 470 nm. The influence of pH, antisera volume, PEG concentration, incubation time and co-exists substances was considered. Under the optimal conditions, the RS intensity is proportional to the concentrations of ApoAI and ApoB when their concentration is in the range of 8.4-430.0 ng·mL-1 and 14.8-590.0 ng·mL-1, respectively. The detection limits (DL) are 6.2 ng·mL-1 for ApoAI and 7.0 ng·mL-1 for ApoB. The method was successfully applied to determination of ApoAI and ApoB in human serum samples, with satisfactory results.
［1］ Rosseneu M, Vercaemst R, Steinberg K K. Clin. Chem., 1983, 29：427. ［2］ Steinberg K K, Cooper G R, Graiser S R. Clin. Chem., 1983, 29：415. ［3］ Contois J H, Mcnamara J R, Lammi-Keefe C J. Clin. Chem., 1996, 42：515. ［4］ Conotois J H, Mcnamara J R, Lammi-Keefe C J. Clin. Chem., 1996, 42：507. ［5］ Kinoshita M, Kijima M, Matsushima T, et al. Clin. Chim. Acta, 2005, 351：115. ［6］ Hartmut M L, Rainer L, Angelika E，et al. J. Chromatograph A, 1995, 717：25. ［7］ Huang C Z, Li Y F. Anal. Chim. Acta, 2003, 500：105. ［8］ Liu S P, Luo H Q, Li N B, et al. Anal. Chem., 2001, 73：3907. ［9］ WEI Yong-ju, QI Xiu-qu, DUN Hui-juan, et al(魏永巨，戚秀菊，敦惠娟, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2005, 25(8)：1289. ［10］ BAI Yan, LI Wei-jia, WU Ya-qin, et al(白 燕，李维嘉，吴雅琴, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2006，26(2)：313. ［11］ Liu S P, Zhang Z Y, Luo H Q. Anal. Sci., 2002, 18：971. ［12］ Jiang Z L, Sun S J, Liang A H, et al. Anal. Chim. Acta, 2006, 571：200. ［13］ LONG Yun-fei, CHEN Xiao-ming, CAI Tie-jun(龙云飞，陈小明，蔡铁军). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2004，24(5)：610. ［14］ Long X F, Bi S P, Ni H Y, et al. Anal. Chim. Acta, 2004, 501(1)：89. ［15］ CHENG Yun-yan, JIANG Zhi-liang, LIANG Ai-hui(程云燕, 蒋治良, 梁爱惠). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2006，26(10)：1888. ［16］ WAN Xin-jun, LIU Jin-shui, WANG Lun(万新军，刘金水，王 伦). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2005，25(5)：754. ［17］ ZHANG Hong-yi, LIU Bao-sheng, ZHANG Hong-lei, et al(张红医, 刘保生, 张红蕾, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2002，22(6)：1054. ［18］ REN Li-ping, JIANG Shu-ren, RAO Zhen-hong, et al(任丽萍, 江树人, 饶震红, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2004, 24(10)：1213. ［19］ Jiang Z L, Zhou S M, Liang A H, et al. Environ. Sci. Technol., 2006, 40：4286. ［20］ Jiang Z L, Sun S J, Liang A H, et al. Clin. Chem., 2006, 52：1389. ［21］ DENG Jun-yao, SUN Shuang-jiao, JIANG Zhi-liang, et al(邓峻耀, 孙双娇, 蒋治良, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2006, 26(8)：1487. ［22］ LI Yong，ZHUANG Yi-yi(李 勇，庄一义). Journal of Clinical Test(临床检验杂志)，1999, 16：333.
