Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Ministry of Education, School of Environment and Resource, Guangxi Normal University, Guilin 541000, China
Abstract:Under the condition of sodium citrate as stabilizer, the gold-selenium (AuSe) nano-alloy was prepared by sodium borohydride reduction procedure, and was modified by single-strand aptamer to obtain an aptamer nano-alloy probe (apta-AuSe) for Hg(Ⅱ). In pH 6.8 Na2HPO4-NaH2PO4 buffer solution and in the presence of NaCl of 33 mmol·L-1, the Apta-AuSe probe is not aggregation. The apta-AuSe interacts with Hg2+ to form stable double-strand T-Hg(Ⅱ)-T mismatches and to release AuSe nano-alloy particles from the probe. The released AuSe nano-alloy particles (20∶1) aggregated to form bigger clusters that resulted in the resonance scattering (RS) intensity (I590 nm) increasing at 590 nm. The increased intensity ΔI590 nm was proportional to the Hg2+ concentration from 1.3 to 1 466 nmol·L-1,with a detection limit of 0.74 nmol·L-1.The regress equation was ΔI590 nm=0.603c+2.0. Thus, a new resonance scattering (RS) spectroscopy of apta-AuSe was applied to the analysis of trace mercury ion. This simple, rapid, selective and sensitive aptamer AuSe nano-alloy RS assay was applied to the determination of Hg2+ in wastewater, with satisfactory results.
[1] Sekowski J W, Malkas L H, Wei Y T, et al. Toxicol. Appl. Pharm., 1997, 145: 268. [2] Coester C J. Anal. Chem., 2005, 77: 3807. [3] Yang Y K, Yook K J, Tae J. J. Am. Chem. Soc. 2005, 127: 16760. [4] Huang C C, Yang Z, Lee K H, et al. Angew Chem Int Ed., 2007, 46: 6824. [5] Xu X H, Thundat T G, Brown G M, et al. Anal. Chem., 2002, 74: 3611. [6] Cheng A K, Ge B, Yu H Z. Anal. Chem., 2007, 79: 5158. [7] Centi S, Messina G, Tombelli S, et al. Biosens. Bioelectron., 2008, 23: 1602. [8] Jiang Z L, Fan Y Y, Chen M L, et al. Anal. Chem., 2009, 81: 5439. [9] Wu L P, Li Y F, Huang C Z, et al. Anal. Chem., 2006, 78: 5570. [10] Jiang Z L, Sun S J, Liang A H, et al. Clin. Chem., 2006, 52: 1389. [11] Long Y F, Huang C Z, Li Y F. J. Phys. Chem. B, 2007, 111: 4535. [12] Zhan G, Zhang L, Li C. Colloid. Surface B, 2009, 71: 84. [13] Cai H H, Yang P H, Feng J, et al. Sensor. Actuat B, 2008, 135: 603. [14] Xu X Y, Georganopoulou D G, Hill H D, et al. Anal. Chem., 2007, 79: 6650. [15] Jiang Z L, Liao X J, Deng A P, et al. Anal. Chem., 2008, 80: 8681. [16] Wei X L, Zou M J, Jiang Z L, et al. Plasmonics, 2008, 3: 73. [17] Li D, Wieckowska A, Willner I. Angew. Chem. Int. Ed., 2008, 47: 3927. [18] Huang C C, Yang Z, Lee K H. Angew. Chem. Int. Ed., 2007, 46: 6824.