Molecular Imprinting-Flow Injection Chemiluminescence Method for Determination of Doxycycline Hydrochloride
ZHOU Yan-mei1, ZHANG Cheng-li1, LEI Jian-du2, MA Tong-sen1, MA Guang-hui2, SU Zhi-guo2, CHAO Yang1
1. College of Chemistry and Chemical Engineering, Henan University, Kaifeng 475004, China 2. National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100080, China
Abstract：Doxycycline hydrochloride can enhance the chemiluminescence of potassium ferricyanide and luminol in alkaline medium. So a molecular imprinting-flow-injection chemiluminescence method for the determination of doxycycline hydrochloride was established by using doxycycline hydrochloride-imprinted polymers as recognition material and potassium ferricyanide and luminol as detection system. Doxycycline hydrochloride-imprinted polymer was synthesized using methacrylic acid as functional monomer and ethylene glycol dimethacrylate as cross-linker. The linear range is 9.0×10-7-6.0×10-5 g·mL-1 and the detection limit is 3.2×10-7 g·mL-1. The relative standard deviation for 6.0×10-6 g·mL-1 of doxycycline hydrochloride was 3.5% (n=9). This method has been successfully applied to the determination of doxycycline hydrochloride in tablets and in urine samples.
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