Abstract：A molecularly imprinted polymers (MIPs) of Ractopamine (RCT) was prepared by thermal polymerization method, and the adsorptive characters of the MIPs was investigated with ultraviolet spectrophotometric method. The results showed that RCT had the maximum absorbance value at the wavelength of 272 nm, the regression equation of RCT was y=7.354 1x+0.001 0, R2=0.999 9, and the average adsorption rate of MIPs was 83.4%. According to the adsorption kinetics, the adsorption time should be controlled within 10 minutes. Infrared spectrum analysis indicated that the MIPs was formed by hydrogen bonds between RCT and functional monomer methacrylic acid, the MIPs of RCT recognized RCT and combined with it exclusively via hydrogen bonds. The investigation is very useful and important for establishing RCT detection methods based on molecularly imprinted technology.
[1] Ross K A, Beaulieu A D, Merrill J, et al. Journal of Animal Science, 2011, 89(7): 2243. [2] Woerner D R, Tatum J D, Engle T E, et al. Journal of Animal Science, 2011, 89(1): 201. [3] Pleadin J, Vulic A, Persi N, et al. Food Technology & Biotechnology, 2011, 49(4): 517. [4] Pleadin J, Persi N, Vulic A, et al. Meat Science, 2012, 90(3): 755. [5] Pleadin J, Vulic A, Persi N, et al. Immunopharmacol Immunotoxicol, 2013, 35(1): 88. [6] Zhang Baixin, Zhao Jingchan, Bijing Sha, et al. Analitical Methods, 2012, 10: 3187. [7] Kan Xianwen, Xing Zonglan, Zhu Anhong, et al. Sensors and Actuators B: Chemical, 2012, 168(20): 395. [8] Yuuki Sueyoshi, Akira Utsunomiya, Masakazu Yoshikawa, et al. Journal of Membrane Science, 2012, 401-402: 89. [9] Chen L X , Xu S F , Li J H . Chemical Society Reviews, 2011, 40: 2922. [10] Yuma Hiratsuka, Noriko Funaya, Hisami Matsunaga, et al. Journal of Pharmaceutical and Biomedical Analysis, 2013, 75: 180. [11] Zeng Huan, Wang Yuzhi, Liu Xiaojie, et al. Talanta, 2012, 93: 172. [12] Xiao Xia Lia, Xin Liua, Li Hong Baia, et al. Chinese Chemical Letters, 2011, 22(8): 989.
