Abstract：An analytical method for the determination of ribonucleic acid by spectrophotometry was established. At the maximum absorption wavelength for neutral red in B-R buffer solution, and under the best conditions, the decrease in the absorbance was linear with the amount of ribonucleic acid. The linearity range was 0.0-9.0 μg·mL-1,the detection limit was 0.52 μg·mL-1,and the correlation coeffient was 0.999 8. This method was simple, rapid, and selective. So its application to the determination of ribonucleic acid was satisfactory. The reaction mechanism was that the electrostatic interaction leads to molecular association of RNA with neutral red, resulting in anti-ion permutation and bonding reaction.
Key words：RNA;Neutral red;Spectroscopy;Electrostatic association
司文会. 核糖核酸与中性红作用机理及含量测定的研究[J]. 光谱学与光谱分析, 2007, 27(06): 1181-1184.
SI Wen-hui. Studies on the Interaction between RNA with Neutral Red and Determination of RNA by Spectrophotometry. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2007, 27(06): 1181-1184.
[1] GUO Pei-xuan(郭培宣). Progress in Natural Science(自然科学进展)，2003，13(8)：785. [2] LIAO Rong-xia, ZHOU Xin-jian(廖容霞, 周新建). Journal of Chemistry of Life(生命的化学)，2002，22(4)：324. [3] Jacque J, Triques K, Stevenson M. Nature, 2002, 418: 435. [4] Wilda M, Fuchs U, Wossmann W, et al. Oncogene, 2002, 21(37): 5716. [5] LU Sheng-dong(卢圣栋). Experimental Techniques of Modern Molecular Biology(现代分子生物学实验技术, 第2版). Beijing：Chinese Union Medical College Press(北京：中国协和医科大学出版社)，1999. 69. [6] LI Liang-zhu，LI Ming-hua, et al(李良铸，李明哗，等). The Newest Technology to Make Biologyical Chemistry Medicine(最新生化药物制备技术). Beijing: Chinese Medicine Press of Science and Technology(北京: 中国医药科技出版社)，2002. 220. [7] XU Jian-qiu(许剑秋). Journal of Grain and Forage Industry(粮食与饲料工业)，1997，(9)：40. [8] YUAN Yu-sun(袁玉荪). Experiment of Biological Chemistry(生物化学实验，第2版). Beijing：Higher Education Press(北京: 高等教育出版社)，1988. 130. [9] FANG Guang-rong，SONG Gong-wu，LI Ling(方光荣, 宋功武, 李 玲). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2002，22(4)：631. [10] WANG Jing-wu，ZHU Xia-ping，SUN Hua, et al(汪敬武, 朱霞萍, 孙 华, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2003，23(5)：899. [11] HUANG Xin-hua，LI Yuan-fang，FENG Ping, et al(黄新华, 李原芳, 奉 萍, 等). Chinese J. Anal. Chem.(分析化学)，2000，28(6)：682. [12] ZHOU Min, MA Yong-jun, ZHANG De-yi, et al(周 敏，马永钧，张德懿，等). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2006，26(9)：1656. [13] CHANG Wen-bao，LI Kei-an(常文保，李克安). Concise Analytical Chemistry Handbook(简明分析化学手册). Beijing: Peking University Press(北京：北京大学出版社)，1981. 264.
