Synthesis and Study on the Interaction of Rare Earth Complexes of N′,N-Bis(2-Pyridinecarboxamide)-1,2-Ethane with DNA
LU Xiao-hong1,LIN Qiu-yue1,2*,HU Rui-ding1,2,LIU Wei-dong1,2,FENG Jie1
1. College of Chemical and Life Science, Zhejiang Normal University, Jinhua 321004，China 2. Zhejiang Key Laboratory for Reaction Chemistry on Solid Surfaces, Zhejiang Normal University, Jinhua 321004, China
Abstract：Four rare earth complexes of N′,N-bis(2-pyridinecarboxamide)-1,2-ethane were synthesized and characterized by elemental analysis, conductivity measurement, thermal studies, IR and electronic spectra. The composition of the four complexes is [Ln(H2L)(NO3)2](NO3)·3H2O (Ln=Sm, Eu, Gd, Tb). Results of spectral measurements indicate that the oxygen of carbonyl and the nitrogen of pyridyl coordinate with Ln(Ⅲ) respectively, and the NO-3 shows bidentate coordination. So the four complexes are 1∶1 chelated complexes. The interaction between [Sm(H2L)(NO3)2](NO3)·3H2O and DNA was studied by employing UV-Visible (UV-Vis) spectra, fluorescence spectra and SERS spectra. Experimental results show that with the incremental addition of DNA, the bands at 265 nm show hypochromism accompanied by a small red shift and the binding constant Kb obtained is 1.24×105. Meanwhile fluorescence spectra show that the addition of [Sm(H2L)(NO3)2](NO3)·3H2O to DNA pretreated with EB causes an appreciable reduction in fluorescence intensity，indicating that the complex competes with ethidium bromide in binding to DNA, and free ethidium bromide increases. The addition of DNA causes the SERS signals of the complex to weaken and the band at 1 282 cm-1 to disappear, which suggests that the planar pyridine molecule of the ligand may partly be inserted into the double-stranded helix plane in DNA, making π electronic density of aromatic rings in complex change. The above phenomena indicate that [Sm(H2L)(NO3)2](NO3)·3H2O interacts intensively with DNA.
陆晓红1,林秋月1,2*,胡瑞定1,2,刘卫东1,2,冯洁1 . 稀土N, N′-双(2-吡啶甲酰胺)-1,2-乙烷配合物合成及与DNA的作用[J]. 光谱学与光谱分析, 2007, 27(06): 1176-1180.
LU Xiao-hong1,LIN Qiu-yue1,2*,HU Rui-ding1,2,LIU Wei-dong1,2,FENG Jie1 . Synthesis and Study on the Interaction of Rare Earth Complexes of N′,N-Bis(2-Pyridinecarboxamide)-1,2-Ethane with DNA. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2007, 27(06): 1176-1180.
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