Determination of 8-Hydroxy-2’-Deoxyguanosine in Urine with Protamine Using Resonance Energy Transfer Fluorescence Quenching by Sensitizing Effect of Triton X-100
MI Xian-wen1,3,WANG Yong-sheng1,2*,XUE Jin-hua1,OUYANG Yun-fu1,YANG Sheng-yuan1,WANG Ying1
1. College of Public Health,Nanhua University,Hengyang 421001,China 2. College of Chemistry and Chemical Engineering,Nanhua University，Hengyang 421001,China 3. Huaihua Medical College,Huaihua 418000,China
Abstract：The optimum conditions for the resonance energy transfer between 8-hydroxy-2’-deoxyguanosine(8-OHdG) and protamine were studied,and a novel method for the determination of 8-OHdG has been developed based on the resonance energy transfer fluorescence quenching effect of protamine. The fluorescence quenching intensity of Ps at 301 nm was proportional to the concentration of 8-OHdG. The linear range of the calibration curve was 0.50-7.1 μg·mL-1 in the pH 8.2 Tris-HCl buffer solution with the detection limits of 0.18 μg·mL-1. The relative standard deviation was 6.2% and the average recovery was 104%(n=6). The proposed method is simple and sensitive,and has succeeded in analyzing the spiked urine sample with satisfactory results.
Key words：8-Hydroxy-2’-deoxyguanosine;Protamine;Fluorescence quenching;Resonance energy transfer
米贤文1,3,王永生1,2*,薛金花1,欧阳运富1,杨胜园1,王英1 . Triton X-100敏化鱼精蛋白共振能量转移荧光猝灭法测定尿中8-羟基脱氧鸟苷[J]. 光谱学与光谱分析, 2007, 27(08): 1570-1573.
MI Xian-wen1,3,WANG Yong-sheng1,2*,XUE Jin-hua1,OUYANG Yun-fu1,YANG Sheng-yuan1,WANG Ying1 . Determination of 8-Hydroxy-2’-Deoxyguanosine in Urine with Protamine Using Resonance Energy Transfer Fluorescence Quenching by Sensitizing Effect of Triton X-100 . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2007, 27(08): 1570-1573.
