Temperature Dependence Study of Calf Thymus DNA by Raman Spectra
DONG Rui-xin1，2, YAN Xun-ling1, WU Jing2, ZHANG An-ying2
1. Department Physics of Liaocheng University, Liaocheng 252059，China 2. University of Electronic Science and Technology of China, Chengdu 610054，China
Abstract：In this paper, the authors obtained Raman spectra of DNA in fiber and aqueous solution at different temperatures. These spectra revealed that the vibration of bases and phosphate group were influenced by varying temperature. Adenine was the most sensitive to varying temperature in all of the vibrational modes. The wave numbers of most of the vibrational modes decreased as the temperature increased except for the band at 1 101 cm-1，and the shift of wave numbers mainly concentrated upon the beginning point of denaturation at 70 ℃. The temperature dependence of Raman intensity was discussed, and two peaks were obtained at 38 and 82 ℃，respectivelｙ. The results obtained by Raman spectra were in agreement with experimental data by DSC at 82 ℃. The peak at 38 ℃ was related to the biological activity region of DNA function.
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