Abstract：Not only dye monomer is the form of energy transfer but also dye dimer is the effective form of energy transfer. In this work, the energy transfer system of acridine orange(AO)-rhodamine B(RB) dimer was built for the determination of DNA as fluorescence probe. At the same time, the mechanism was also considered. Under the optimum experimental conditions, the linear range of this assay was 0.33-1.33 mg·L-1 for fish sperm DNA and 0.33-3.33 mg·L-1 for calf thymus DNA, the detection limit was 1.63×10-3 mg·L-1 for fish sperm DNA and 1.52×10-3 mg·L-1 for calf thymus DNA, respectively. The RSD for the determination of 1.00 mg·L-1 DNA was 2.40% for fsDNA and 2.00% for ctDNA, respectively. The method was used for the determination of DNA in synthetic samples with satisfied results.
高 峰，朱昌青，王 伦* . 吖啶橙-罗丹明B二聚体能量转移体系在DNA测定中的应用及机理研究 [J]. 光谱学与光谱分析, 2004, 24(01): 85-88.
GAO Feng, ZHU Chang-qing, WANG Lun . Studies on Application and Mechanism of Energy Transfer System of Acridine Orange(AO)-Rhodamine B(RB) Dimer in the Determination of DNA . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2004, 24(01): 85-88.
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