Abstract：Both Zhenbao pills Ⅱ and Ⅲ are Mongolian medicine of a ｋind． Zhenbao pill Ⅱ was the artificial syntheses and Zhenbao pill Ⅲ was made from natural materials． In this paper，the flavonoids of Mongolian medicine Zhenbao pills Ⅱ and Ⅲ were eｘtracted by Soｘhlet apparatus with methyl alcohol as eｘtracting agent，the colorimetric method was applied to the determination of flavonoids，and the eｘperimental procedure was studied with Zhenbao pill Ⅱ as the test sample． The result showed that the linear range of quantitative determination was 8.05-48.28 μg·mg-1. The standard addition recovery(SAR) was 99.49%-100.50%. The RSD(n=3) was 0.54%.The range of contents of flavonoids was 1.47-1.55 mg·g-1 in Zhenbao pill Ⅱ and was 2.88-3.00 mg·g-1 in Zhenbao pill Ⅲ. This method was simple and accurate with good reproducibility，and is suitable for the determination of flavonoids in all ｋinds of pills． The contents of flavonoids can be used to prove whether Zhenbao pill is artificial syntheses or natural material.
Key words：Colorimetric method;Zhenbao pills Ⅱ and Ⅲ;Flavonoids
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