Abstract：Microwave digestion technique was used in the decomposition of Ginkgo biloba leaves from six different trees at the same age in the same area. HNO3-H2O2 (5∶1 v/v) was used as microwave digestion agent at a suitable temperature and time. The contents of Ca, Mg, K, Na, Cu, Zn and Zn/Cu were determined by flame atomic absorption spectrometry to study the distribution rule of metallic elements in the trees at the same age and in the same area. The recovery ratio ranged from 95.2% to 104.6%. The results showed that there were certain differences between different trees in the distribution of metallic elements. The contents of calcium were from 39 586 to 48 320 μg·g-1, and those of magnesium from 10 076 to 12 918 μg·g-1, of potassium from 2 004 to 5 240 μg·g-1, of sodium from 9.05 to 35.30 μg·g-1, and of copper from 1.50 to 3.05 μg·g-1, while Zn/Cu values were from 2.68 to 5.93 in the leaves of 6 different trees in the same growing area. Therefore there were abounding calcium, magnesium and potassium, while the content of sodium and Zn/Cu values were lower, and the metal contents were different in the leaves. The experimental results provided useful bases for studying the distribution rule of metallic elements in Ginkgo biloba leaves, the relationship between the contents of calcium, magnesium, potassium and sodium and the Zn/Cu value in ginkgo biloba leaves and the treatment for cardio-cerebral vascular disease.
[1] Laura L Boles Ponto. PhD Susan K Schultz. MD. Annals of Clinical Psychiatry, 2003, 15(2): 109. [2] Sung Y Won Jae Choi, Jong Moon Park，Yang Ji-won. Biotecknology Teckniques，1997, 11(8): 553. [3] XIAO Pei-gen(肖培根). Modern Chinese Materia Medica, Vol. 3(新编中药志，第3卷). Beijing: Chemical Industry Press(北京：化学工业出版社)，2002. 491. [4] JIANG Bo(姜 波). Lubricating Oil(润滑油). 1998, 13(4): 52. [5] E Er-deng-sang, BA Tu, JIA Mei-lin, et al(额尔登桑，巴 图，贾美林，等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2008, 28(6): 1394. [6] GAO Yan, WANG Zhi-lun, KAO Xi-bin(高 艳，王治伦，考希宾). Chin. J. Ctrl. Endem. Dis.(中国地方病防治杂志), 2007, 22(4): 268. [7] MU Jian-jun, LIU Zhi-quan, YANG Jun, et al(牟建军，刘治全，杨 军，等). Chin. J. Prev. Med.(中国预防医学杂志), 2003, 37(2): 90. [8] DING Ye, WANG Feng, ZHANG Wen-bin, et al(丁 烨，王 枫，张文斌，等). Chinese Journal of Clinical Rehabilitation(中国临床康复), 2006, 10(48): 86. [9] WANG Yun-ming, WAN Jin-zhong(王允明，万金中). Modern Practical Medicine(现代实用医学), 2008, 20(8): 618. [10] ZHANG Yu-dou, YUAN Xia(张玉斗，袁 霞). Clinical Focus(临床荟萃), 2003, 18(19): 1137. [11] ZHANG Jie(张 洁). Foreign Medical Sciences (Section of Medgeography)(国外医学医学地理分册), 2004, 25(4): 156. [12] HUANG Zhi-long(黄之龙). Anthology of Medicine(医学文选), 2003, 22(4): 473. [13] DANG Jing-dan(党京丹). Medical Recapitulate(医学综述), 2006, 12(19): 1181. [14] MA Wei-juan(马玮娟). Foreign Medical Sciences (Section of Medgeography)(国外医学医学地理分册), 2006, 27(4): 164. [15] YUAN Bo-yong, QIN Qing-yun, JIA Xiang-lan(袁伯勇，秦庆云，郏香兰). Studies of Trace Elements and Health(微量元素与健康研究), 1994, 11(4): 32. [16] HAI Li, LI Xi-chang, ZHAO Wen-ying, et al(海 黎，李喜昌，赵文颖，等). Inner Mongol Journal of Traditional Chinese Medicine(内蒙古中医药)，1995，14(1): 37. [17] HU Xue-mei, TAN Guang-qun, LI Hui(胡雪梅，谭光群，李 晖). Journal of Sichuan University(Engineering Science Edition)(四川大学学报·工程科学版), 2003, 35(1): 109. [18] WEI Cai-xia, YANG Jing, CAI Hu(魏彩霞，杨 静，蔡 虎). Digest of the World Latest Medical Information(世界最新医学信息文摘), 2004, 3(5): 1319.
