茶树叶与根表面的XPS表征

doi:10.3964/j.issn.1000-0593(2008)09-2196-05

Abstract
References
Related Citation (15)

Download: PDF (1760 KB)
Export: BibTeX | EndNote (RIS)

Abstract XPS was applied to analyze the surface chemical composition and structure of the tea plant leaf and root. It was detected that the surface is made up of mainly 4 elements：C, O, N and Al, with little P and F in abaxial leaf. Based on the botanic epidermis structure and the chemical composition, with the help of the standard spectrum data bank on line and the wood XPS study results, and through line Gaussian and Lorentizian the mixed, the binding energy of C（1s） of the leaf surface was classified as 3 types: the first was C1, with the electron binding energy of 285 eV, from C—C or C—H group, representing lipid compound like cutin and wax. C2 with the binding energy of 286.35 eV in the adaxial and 286.61 eV in the abaxial, came from the single bond of carbon and oxygen C—O, mainly standing for cellulose. C3 with the binding energy of approximately 288 eV (288.04 eV in adaxial and 288.09 eV in abaxial) was the sign of CO group, which is acyl in protein with the confirmation of N（1s）（399-401 eV）and O（1s） analyses. In the root surface, besides the same compounds of cutin and wax (C1, binding energy 285 eV), cellulose (C2, binding energy 286.49 eV) and protein （C3，binding energy 288.78 eV）as in the leaf, there appeared C5 type with the binding energy of 283.32 eV . Because it was lower than C1, it was estimated as carbon linking to metal. Both the leaf and the root surfaces didn’t have C4, a type of OCO, which is common in wood surface with the highest oxidated carbon of 289-289.5 eV binding energy, indicating that organic acid secreted by the root existed freely on the root surface, without any chemical association with the surface compounds. The results of the separated spectrum of O（1s） supported the above C（1s） results. By the ratio of each type of C, there were more oxygen groups in the abaxial than in the adaxial, implicating more active chemical properties on the abaxial. Compared with the leaf, cutin and wax was little in the root and oxygen groups were many, verifying more active chemical property on the root surface and more water and solute molecules passing. Again the protein content was in the order of root, abaxial and adaxial, indicating the same order of the wetness degree. Higher binding energy of Al than 73.50 eV showed oxidized aluminum in tea plant surface, which might enhance the absorption, and more oxidized aluminum in the root meants that it has more powerful absorbability.

Key words：Tea plant;Leaf;Root;Surface;XPS

Received: 2006-11-16 Accepted: 2007-02-18

ZTFLH:

Q94-334

Corresponding Authors: WAN Xiao-chun E-mail: xcwan@ahau.edu.cn

Cite this article:

FANG Jiang-yu,WAN Xiao-chun. XPS Analysis of Tea Plant Leaf and Root Surface[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2008, 28(09): 2196-2200.

URL:

https://www.gpxygpfx.com/EN/10.3964/j.issn.1000-0593(2008)09-2196-05 OR https://www.gpxygpfx.com/EN/Y2008/V28/I09/2196

[1] Hunsche M, Bringe K, Schmitz-Eiberger M, et al. PEST Mangement Science, 2006, 62(9): 839.
[2] Kirkwood C R. Pesticide Science, 1999, 55(1): 69.
[3] ZHANG Hao, ZHUANG Xue-ying（张浩, 庄雪影）. Journal of South China Agricultural University(华南农业大学学报), 2004, 25(3): 87.
[4] AO Cheng-qi, CHEN Gong-xi, ZHANG Hong-da(敖成齐, 陈功锡, 张宏达). Acta Botanica Yunnanica(云南植物研究), 2002, 24(1): 68.
[5] WANG Ren-xiang, HU Chang-hua, LIANG Sheng-ye, et al(王任翔, 胡长华, 梁盛业, 等). Guangxi Forestry Science(广西林业科学), 2002, 31(3): 133.
[6] WANG Ren-xiang, LIANG Sheng-ye, LI Jie-rong, et al(王任翔, 梁盛业, 李洁荣, 等). Guangxi Forestry Science（广西林业科学）, 2003, 32(1): 12.
[7] LI Feng-ying, WANG Yu-guo, TANG Shao-qing(李凤英, 王玉国, 唐绍清). Journal of Guangxi Normal University(Natural Science)(广西师范大学学报·自然科学版), 2001, 19(4): 75.
[8] Fang Jiangyu, Zhang Fusuo, Zhang Wenxu, et al. Nanostructural Silica in Rice and Properties Beneficial to the Plant. Plant Nutrition for Food Security, Human Health and Environmental Protection. The 15th International Plant Nutrition Colloqnium. Beijing: Tsinghua University Press, 2005. 278.
[9] LIN Guan-fa, ZHENG Mao-sheng, BAI Zhen-quan, et al(林冠发, 郑茂盛, 白真权, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2005, 25(11): 1875.
[10] XU Yi, TANG Shou-yuan, ZHANG Xiao-feng(徐溢, 唐守渊, 张晓凤). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2004, 24(4): 495.
[11] Hua X, Kaliaguine S, Kokta B V, et al. Wood Science and Technology, 1993, 27: 449.
[12] DU Guan-ben(杜官本). China Wood Industry(木材工业), 1999, 13(3): 17.
[13] YANG Xi-kun, DU Guan-ben, QIAN Tian-cai, et al(杨喜昆, 杜官本, 钱天才, 等). Journal of Instrumental Analysis(分析测试学报), 2003, 22(4): 6.
[14] Allebone J E, Hamilton R J. Journal of the Science of Food and Agriculture, 1972, 23(6): 777.
[15] Barik A, Bhattacharya B, Laskar S, et al. Phytochemical Analysis, 2004, 15(2): 109.
[16] Prügel B, Lognay G. Phytochemical Analysis, 1996, 7(1): 29.
[17] YE Qing-hua, ZENG Ding, CHEN Zhen-duan, et al(叶庆华, 曾定, 陈振端, 等). Botanic Biology(植物生物学). Xiamen: Xiamen University Press(厦门: 厦门大学出版社), 2005. 64.
[18] ZHANG Feng-juan, CHEN Feng-xin, XU Xing-you, et al(张风娟, 陈凤新, 徐兴友, 等). Acta Pratacultural Science(草业科学), 2006, 23(9): 19.
[19] ZHAO Qing-fang, MA Rui-jun, DU Guo-zhen, et al(赵庆芳, 马瑞君, 杜国桢, 等). Journal of Lanzhou University (Natural Sciences)(兰州大学学报·自然科学版), 2006, 42(1): 33.
[20] LI Fang-lan, BAO Wei-kai, LIU Jun-hua, et al(李芳兰, 包维楷, 刘俊华, 等). Chinese Journal of Applied Ecology(应用生态学报), 2006, 17(1): 5.
[21] ZHU Ming-wei, WANG Hong-xing, LI Jian-jun, et al(朱命炜, 王洪星, 李建军, 等). Journal of Chinese Electron Microscopy Society(电子显微学报), 2004, 23(6): 670.
[22] XPS(X射线光电子能谱).http://2002.38.64.11/~mams/escalab/Lects.pdf.
[23] The NIST X-ray Photoelectron Spectroscopy (XPS) Database. http://srdata.nist.gov/xps.
[24] LIN Jian-min, YANG Peng-yuan, WANG Xiao-ru, et al(林建民, 杨原, 王小如, 等). Chinese Journal of Analytical Laboratory(分析试验室), 1994, 13(3): 6.