光谱学与光谱分析 |
|
|
|
|
|
Structure and Anti-Tumor Activity of Bovine α-Lactalbumin after Binding Linoleic Acid |
ZHANG Ming1, FANG Bing3, ZHANG Lu-da2, REN Fa-zheng2* |
1. Beijing Technology and Business University, Beijing 100048, China 2. Key Laboratory of Functional Dairy, China Agricultural University, Beijing 100083, China 3. Academy of State Administration of Grain, Beijing 100037, China |
|
|
Abstract The structure changes of α-lactalbumin after binding oleic acid and linoleic acid, including hydrophobic amino acids, hydrophobic regions, tertiary structure, secondary structure, was studied by intrinsic fluorescence, ANS-binding intrinsic fluorescence and circular dichroism spectrum, respectively. The anti-tumor activity of the lactalbumin-oleic acid complex and lactalbumin-linoleic acid complex was measured using the methylene blue method. It can be seen from the fluorescence spectra that a significant red-shift from 331.07 to 337.67 nm and 337.60 nm of α-lactalbumin occurred after binding of oleic acid and linoleic acid, respectively. Together with the ANS-binding spectra, which exhibited a blue-shift (from 516.20 to 514.10 nm and 508.50 nm, respectively) with an increased fluorescence intensity, it can be indicated that binding of oleic acid and linoleic acid lead to the exposure of hydrophobic amino acids and hydrophobic regions. Results of circular dichroism spectra indicated the partial loss of the tertiary structure, and an decrease of β-turn and random coil, which turn to the β-sheet structure. Furthermore, the anti-tumor activity of the two complexes was verified on the three types of tumor cells. This study laid a theoretical basis for the development of anti-tumor drugs.
|
Received: 2014-08-25
Accepted: 2014-11-22
|
|
Corresponding Authors:
REN Fa-zheng
E-mail: renfazheng@263.net
|
|
[1] Hakansson A P, Roche-Hakansson H, Mossberg A K, et al. PLoS One, 2011, 6(3): e17717. [2] Svensson M, Hakansson A, Mossberg A K, et al. Proc Natl. Acad. Sci. USA, 2000, 97(8): 4221. [3] Svensson M, Fast J, Mossberg A K, et al. Protein Science, 2003, 12(12): 2794. [4] Fischer W, Gustafsson L, Mossberg A K, et al. Cancer Research, 2004, 64(6): 2105. [5] Mossberg A K, Wullt B, Gustafsson L, et al. International Journal of Cancer, 2007, 121(6): 1352. [6] Mossberg A K, Hou Y C, Svensson M, et al. Journal of Urology, 2010, 183(4): 1590. [7] Gustafsson L, Leijonhufvud I, Aronsson A, et al. New England Journal of Medicine, 2004, 350(26): 2663. [8] Brinkmann C R, Heegaard C W, Petersen T E, et al. Febs Journal, 2011, 278(11): 1955. [9] Nakamura T, Aizawa T, Kariya R, et al. Journal of Biological Chemistry, 2013, 288(20): 14408. [10] Permyakov S E, Pershikova I V, Zhadan A P, et al. Journal of Proteome Research, 2005, 4(2): 564. [11] Fast J, Mossberg A K, Svanborg C, et al. Protein Science, 2005, 14(2): 329. [12] Eftink M R. Biophysical Journal, 1994, 66(2): 482. [13] Eftink M R, Shastry M C R. Fluorescence Spectroscopy, 1997, 278: 258. [14] Pastukhov A V, Ropson I J. Proteins-Structure Function and Genetics, 2003, 53(3): 607. |
[1] |
WANG Xiao-xia1*, NIE Zhi-hua2, MA Li-tong1, CUI Jin-long1, SAI Hua-zheng1,ZHAO Wen-yuan1. Study on the Interaction Between Minocycline and Bovine Serum Albumin by Multi Spectral Method and Molecular Docking Simulation[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(05): 1503-1508. |
[2] |
HUANG Chao-bo3, XU Han3, YANG Ming-guan3, LI Zhen-jing1, 3, YANG Hua1, 3, WANG Chang-lu1, 2, 3, ZHOU Qing-li1, 2, 3*. The Interaction between Rubropunctamine and Bovine Serum Albumin Using Spectrometry and Molecular Docking[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2019, 39(10): 3102-3108. |
[3] |
SUN Ning-jie1, CAI Xiao-li1, ZHANG Yue-fei1, CHI Ru-an1*, Yunjie Xu2*. The Conformation of Sophocarpidin by Vibrational Circular Dichroism and DFT Calculations[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(09): 2806-2813. |
[4] |
WANG Hao1, 2, HUANG Yan2, LIU Zhi-tong2, GAO Zhen-hua2, SUN Da-rui2, TAO Ye2*. Development of Dynamic Synchrotron Radiation Circular Dichroism Spectroscopy Using Continuous-Flow Probe with Microfluidic Mixer[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2017, 37(07): 2110-2114. |
[5] |
CAI Jian-zhou1, ZHANG Wu2* . Studies on the Interaction of Poly-L-lysine with Fibrinogen with Spectroscopic Methods[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2017, 37(01): 166-171. |
[6] |
HU Tao-ying1, WANG Yi-run1, ZHOU Shan-shan1,2, LIU Ying1,2* . Investigation of the Interaction between Perfluorododecanoic Acid and Human Serum Albumin by Multi-Spectroscopic and Molecular Modeling Techniques[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2016, 36(07): 2330-2336. |
[7] |
CAO Juan1, HUANG Yan1*, ZHANG Qi1, 2*, HUA Yu-jie1, HUA Ming-qing1, GONG Jian-bo1, ZHU Wei-hua1 . Study on the Interaction between Low Molecular Weight Chitosan-Based Schiff Bases and Bovine Serum Albumin [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2016, 36(04): 1090-1094. |
[8] |
WU Shu-rong2, LIU Ying1, LIU Shu-fang1* . Spectroscopic Studies on the Interaction of Human Serum Albumin and Water-Soluble Carboxyl Carbon Nanotubes[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2016, 36(04): 1109-1115. |
[9] |
WEN Shuai, MA Ying-xuan, WU Kun-sheng, CUI Jin-tian, LUO Yun-bo, QU Gui-qin* . Research on the Tomato Metacaspase Protein Interactions with Ca2+ by Spectroscopy and Molecular Probe [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2015, 35(06): 1643-1648. |
[10] |
YANG Wei, YUAN Fang, GAO Yan-xiang* . Interaction of Fish Collagen Peptide with Epigallocatechin Gallate [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2015, 35(01): 184-188. |
[11] |
XI Jia-fu1, TANG Lei1*, ZHANG Jian-hua2, ZHANG Hong-jian2, CHEN Xu-sheng2, MAO Zhong-gui2 . Characterization of Structural Change of Ascorbate Peroxidase from Chinese Kale during Denaturation by Circular Dichroism[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2014, 34(11): 3062-3065. |
[12] |
ZHANG Zhi-bao1, WANG Jing-jie1, CHEN Hui-juan1, XIONG Qing-qing1, LIU Ling-rong1*, ZHANG Qi-qing1,2* . Study of Collagen Mimetic Peptide’s Triple-Helix Structure and Its Thermostability by Circular Dichroism[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2014, 34(04): 1050-1055. |
[13] |
ZHANG Qi-bing, NA Xin-zhu, YIN Zong-ning* . Characterization of Thermal Denaturation Process of Proteinase K by Spectrometry [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2013, 33(07): 1749-1753. |
[14] |
SHI Xiao-xia, MA Mei-hu*, WANG Li-ying, HUANG Xi. Effect of Heat Treatment on the Allergenicity and Microstructure of Ovomucoid [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2012, 32(09): 2487-2491. |
[15] |
HUANG Qun1, 2,MA Mei-hu1*,CAI Zhao-xia1,LUO Zhang1, 3,HUANG Xi1,SUN Shu-guo1, 2 . Effect of S-Configuration Transformation on the Microstructure of Ovalbumin [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2011, 31(12): 3319-3322. |
|
|
|
|